| Platelet-activating factor receptor initiates contact of Acinetobacter baumannii expressing phosphorylcholine with host cells. | |
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MedLine Citation:
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PMID: 22689572 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Adhesion is an initial and important step in Acinetobacter baumannii causing infections. However, the exact molecular mechanism of such a step between A. baumannii and the host cells remains unclear. Here, we demonstrated that the phosphorylcholine (ChoP)-containing outer membrane protein of A. baumannii binds to A549 cells through platelet-activating factor receptor (PAFR), resulting in activation of G protein and intracellular calcium. Upon A. baumannii expressing ChoP binding to PAFR, clathrin and β-arrestins, proteins involved in the direction of the vacuolar movement, are activated during invasion of A. baumannii. PAFR antagonism restricts the dissemination of A. baumannii in the pneumonia model. These results define a role for PAFR in A. baumannii interaction with host cells and suggest a mechanism for the entry of A. baumannii into the cytoplasm of host cells. |
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Authors:
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Younes Smani; Fernando Docobo-Pérez; Rafael López-Rojas; Juan Domínguez-Herrera; José Ibáñez-Martínez; Jerónimo Pachón |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2012-06-11 |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 287 ISSN: 1083-351X ISO Abbreviation: J. Biol. Chem. Publication Date: 2012 Aug |
Date Detail:
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Created Date: 2012-08-06 Completed Date: 2012-11-05 Revised Date: 2013-04-16 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: United States |
Other Details:
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Languages: eng Pagination: 26901-10 Citation Subset: IM |
Affiliation:
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Clinic Unit of Infectious Diseases, Microbiology and Preventive Medicine, Institute of Biomedicine of Seville (IBiS), University Hospital Virgen del Rocío/CSIC/University of Seville, 41013 Seville, Spain. y_smani@hotmail.com |
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| MeSH Terms | |
Descriptor/Qualifier:
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Acinetobacter baumannii
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metabolism* Arrestins / metabolism Bacterial Adhesion Cell Line Clathrin / metabolism GTP-Binding Proteins / metabolism Humans Phosphorylcholine / metabolism* Platelet Membrane Glycoproteins / metabolism* Receptors, G-Protein-Coupled / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Arrestins; 0/Clathrin; 0/Platelet Membrane Glycoproteins; 0/Receptors, G-Protein-Coupled; 0/beta-arrestin; 0/platelet activating factor receptor; 107-73-3/Phosphorylcholine; EC 3.6.1.-/GTP-Binding Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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