Document Detail

Platelet-activating factor receptor initiates contact of Acinetobacter baumannii expressing phosphorylcholine with host cells.
MedLine Citation:
PMID:  22689572     Owner:  NLM     Status:  MEDLINE    
Adhesion is an initial and important step in Acinetobacter baumannii causing infections. However, the exact molecular mechanism of such a step between A. baumannii and the host cells remains unclear. Here, we demonstrated that the phosphorylcholine (ChoP)-containing outer membrane protein of A. baumannii binds to A549 cells through platelet-activating factor receptor (PAFR), resulting in activation of G protein and intracellular calcium. Upon A. baumannii expressing ChoP binding to PAFR, clathrin and β-arrestins, proteins involved in the direction of the vacuolar movement, are activated during invasion of A. baumannii. PAFR antagonism restricts the dissemination of A. baumannii in the pneumonia model. These results define a role for PAFR in A. baumannii interaction with host cells and suggest a mechanism for the entry of A. baumannii into the cytoplasm of host cells.
Younes Smani; Fernando Docobo-Pérez; Rafael López-Rojas; Juan Domínguez-Herrera; José Ibáñez-Martínez; Jerónimo Pachón
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-06-11
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  287     ISSN:  1083-351X     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-08-06     Completed Date:  2012-11-05     Revised Date:  2013-08-14    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  26901-10     Citation Subset:  IM    
Clinic Unit of Infectious Diseases, Microbiology and Preventive Medicine, Institute of Biomedicine of Seville (IBiS), University Hospital Virgen del Rocío/CSIC/University of Seville, 41013 Seville, Spain.
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MeSH Terms
Acinetobacter baumannii / metabolism*
Arrestins / metabolism
Bacterial Adhesion
Cell Line
Clathrin / metabolism
GTP-Binding Proteins / metabolism
Phosphorylcholine / metabolism*
Platelet Membrane Glycoproteins / metabolism*
Receptors, G-Protein-Coupled / metabolism*
Reg. No./Substance:
0/Arrestins; 0/Clathrin; 0/Platelet Membrane Glycoproteins; 0/Receptors, G-Protein-Coupled; 0/beta-arrestin; 0/platelet activating factor receptor; 107-73-3/Phosphorylcholine; EC 3.6.1.-/GTP-Binding Proteins

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