| Platelet-activating factor and evidence of oxidative stress in the bronchoalveolar fluid of Thoroughbred colts during race training. | |
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MedLine Citation:
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PMID: 20102491 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Inflammatory airway disease (IAD) is prevalent in young racehorses during training, being the 2nd most commonly diagnosed ailment interrupting training of 2-year-old Thoroughbred racehorses. HYPOTHESIS: That stabling and exercise cause oxidative stress, release of platelet-activating factor (PAF) and inflammation in airways of Thoroughbred colts. ANIMALS: Colts in breeding farms (NC, n = 45), stabled for 30 days (EC, n = 40), and race trained (EX, n = 34). METHODS: Cytological profile and parameters of bronchoalveolar lavage fluid (BALF) related to oxidative stress, bioactivity of the proinflammatory mediator PAF, catalase activity, and alveolar macrophage function. RESULTS: Percentages of neutrophils and eosinophils in the BALF of the EX group were higher (5.4 +/- 6.4% versus 0.9 +/- 1.2%) than the upper limits for normal horses (3-5%). BALF from the EX group (45.6 +/- 2.8 cells/microL of BALF) also displayed significantly (P = .017) higher total nucleated cell count. PAF bioactivity and the total protein concentration in the BALF were higher in the EX group (0.0683 +/- 0.076 versus 0.0056 +/- 0.007 340 : 380 nm ratio P = .0039, 0.36 +/- 0.30 versus 0.14 +/- 0.15 mg of proteins/mL of BALF P < .001). Concentration of BALF hydroperoxides was higher in the EC group (104.7 +/- 80.0 versus 35.2 +/- 28.0 nmol/mg of proteins, P = .013) and catalase activity was higher in the EX group (0.24 +/- 0.16 versus 0.06 +/- 0.02 micromol H2O2/min/mg of proteins, P = .0021). Alveolar macrophage phagocytosis (P = .048) as well as production of superoxide anion (P = .0014) and hydrogen peroxide (P = .0011) were significantly lower in EX group. CONCLUSIONS AND CLINICAL IMPORTANCE: Further studies should be performed to elucidate the role of PAF in the pathophysiology of IAD. Its presence in bronchoalveolar fluid of young athletic horses makes it a potential therapeutic target to be investigated. |
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Authors:
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P V Michelotto; L A Muehlmann; A L Zanatta; E W R Bieberbach; L C Fernandes; A Nishiyama |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of veterinary internal medicine / American College of Veterinary Internal Medicine Volume: 24 ISSN: 0891-6640 ISO Abbreviation: J. Vet. Intern. Med. Publication Date: 2010 Mar-Apr |
Date Detail:
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Created Date: 2010-05-17 Completed Date: 2010-06-03 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8708660 Medline TA: J Vet Intern Med Country: United States |
Other Details:
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Languages: eng Pagination: 414-9 Citation Subset: IM |
Affiliation:
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Setor de Ci?ncas Biol?gicas, Universidade Federal do Paran? (UFPR), Curitiba, Paran?, Brazil. michelottojunior@yahoo.com.br |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bronchoalveolar Lavage Fluid / chemistry*, cytology Horses / physiology* Macrophages, Alveolar / physiology Male Oxidative Stress / physiology* Physical Conditioning, Animal Platelet Activating Factor / analysis*, metabolism Respiratory System Sports |
| Chemical | |
Reg. No./Substance:
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0/Platelet Activating Factor |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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