Document Detail

Plastocyanin is encoded by an uninterrupted nuclear gene in spinach.
MedLine Citation:
PMID:  2834087     Owner:  NLM     Status:  MEDLINE    
Plastocyanin is a member of photosynthetic electron transport chains that transfers electrons from cytochrome f to the oxidized P700 chlorophyll a pigment of the photosystem I reaction center. We have isolated and characterized cDNA- and genomic clones from spinach (Spinacia oleracea) encoding the complete plastocyanin-precursor polypeptide. The amino acid sequence derived from the nucleotide sequence shows that the precursor consists of 168 amino acid residues including a transit sequence of 69 residues. The precursor polypeptide has a predicted Mr of 16,917, the mature protein of 10,413. The available data indicate that plastocyanin derives probably from a single-copy gene. The coding region contains no intron. The size of the mRNA as determined by S1 nuclease protection experiments is approximately 660 nucleotides, although analysis of different cDNA clones suggests that longer RNA species do exist, approaching the size of the mRNA (850 bases) estimated by Northern blot techniques.
C Rother; T Jansen; A Tyagi; J Tittgen; R G Herrmann
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Current genetics     Volume:  11     ISSN:  0172-8083     ISO Abbreviation:  Curr. Genet.     Publication Date:  1986  
Date Detail:
Created Date:  1988-06-03     Completed Date:  1988-06-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8004904     Medline TA:  Curr Genet     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  171-6     Citation Subset:  IM    
Botanisches Institut, Ludwig-Maximilians-Universität, München, Federal Republic of Germany.
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MeSH Terms
Amino Acid Sequence
Base Sequence
Cell Nucleus / metabolism*
Cloning, Molecular
DNA / genetics,  isolation & purification
DNA Restriction Enzymes
Molecular Sequence Data
Plant Proteins / genetics*
Plants / genetics*
Plastocyanin / genetics*
Reg. No./Substance:
0/Plant Proteins; 9007-49-2/DNA; 9014-09-9/Plastocyanin; EC 3.1.21.-/DNA Restriction Enzymes

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