| Plasticity of the mitoproteome to nitrogen sources (nitrate and ammonium) in Chlamydomonas reinhardtii: the logic of Aox1 gene localization. | |
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MedLine Citation:
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PMID: 20211595 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Nitrate and ammonium constitute primary inorganic nitrogen sources that can be incorporated into carbon skeletons in photosynthetic eukaryotes. In Chlamydomonas, previous studies and the present one showed that the mitochondrial AOX is up-regulated in nitrate-grown cells in comparison with ammonium-grown cells. In this work, we have performed a comparative proteomic analysis of the soluble mitochondrial proteome of Chlamydomonas cells growth either on nitrate or ammonium. Our results highlight important proteomics modifications mostly related to primary metabolism in cells grown on nitrate. We could note an up-regulation of some TCA cycle enzymes and a down-regulation of cytochrome c1 together with an up-regulation of l-arginine and purine catabolism enzymes and of ROS scavenging systems. Hence, in nitrate-grown cells, AOX may play a dual role: (1) lowering the ubiquinone pool reduction level and (2) permitting the export of mitochondrial reducing power under the form of malate for nitrate and nitrite reduction. This role of AOX in the mitochondrial plasticity makes logical the localization of Aox1 in a nitrate assimilation gene cluster. |
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Authors:
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Stéphanie Gérin; Grégory Mathy; Arnaud Blomme; Fabrice Franck; Francis E Sluse |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-03-06 |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 1797 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 2010 Jun-Jul |
Date Detail:
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Created Date: 2010-06-21 Completed Date: 2011-01-10 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: Netherlands |
Other Details:
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Languages: eng Pagination: 994-1003 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier B.V. All rights reserved. |
Affiliation:
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Laboratory of bioenergetics and cellular physiology, B6, Allée de la Chimie 3, 4000 Liège, Belgium. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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metabolism Chlamydomonas reinhardtii / genetics*, metabolism* Citric Acid Cycle Electron Transport Free Radical Scavengers / metabolism Genes, Plant Mitochondria / metabolism Models, Biological Multigene Family Nitrates / metabolism Nitrites / metabolism Nitrogen / metabolism* Oxidative Phosphorylation Oxidoreductases / genetics*, metabolism* Plant Proteins / biosynthesis Proteome / metabolism Purine Nucleotides / metabolism Quaternary Ammonium Compounds / metabolism Reactive Nitrogen Species / metabolism Reactive Oxygen Species / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Free Radical Scavengers; 0/Nitrates; 0/Nitrites; 0/Plant Proteins; 0/Proteome; 0/Purine Nucleotides; 0/Quaternary Ammonium Compounds; 0/Reactive Nitrogen Species; 0/Reactive Oxygen Species; 7727-37-9/Nitrogen; EC 1.-/Oxidoreductases; EC 1.-/alternative oxidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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