Document Detail

Plasticity of the mitoproteome to nitrogen sources (nitrate and ammonium) in Chlamydomonas reinhardtii: the logic of Aox1 gene localization.
MedLine Citation:
PMID:  20211595     Owner:  NLM     Status:  MEDLINE    
Nitrate and ammonium constitute primary inorganic nitrogen sources that can be incorporated into carbon skeletons in photosynthetic eukaryotes. In Chlamydomonas, previous studies and the present one showed that the mitochondrial AOX is up-regulated in nitrate-grown cells in comparison with ammonium-grown cells. In this work, we have performed a comparative proteomic analysis of the soluble mitochondrial proteome of Chlamydomonas cells growth either on nitrate or ammonium. Our results highlight important proteomics modifications mostly related to primary metabolism in cells grown on nitrate. We could note an up-regulation of some TCA cycle enzymes and a down-regulation of cytochrome c1 together with an up-regulation of l-arginine and purine catabolism enzymes and of ROS scavenging systems. Hence, in nitrate-grown cells, AOX may play a dual role: (1) lowering the ubiquinone pool reduction level and (2) permitting the export of mitochondrial reducing power under the form of malate for nitrate and nitrite reduction. This role of AOX in the mitochondrial plasticity makes logical the localization of Aox1 in a nitrate assimilation gene cluster.
Stéphanie Gérin; Grégory Mathy; Arnaud Blomme; Fabrice Franck; Francis E Sluse
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-03-06
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1797     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:    2010 Jun-Jul
Date Detail:
Created Date:  2010-06-21     Completed Date:  2011-01-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  994-1003     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier B.V. All rights reserved.
Laboratory of bioenergetics and cellular physiology, B6, Allée de la Chimie 3, 4000 Liège, Belgium.
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MeSH Terms
Amino Acids / metabolism
Chlamydomonas reinhardtii / genetics*,  metabolism*
Citric Acid Cycle
Electron Transport
Free Radical Scavengers / metabolism
Genes, Plant
Mitochondria / metabolism
Models, Biological
Multigene Family
Nitrates / metabolism
Nitrites / metabolism
Nitrogen / metabolism*
Oxidative Phosphorylation
Oxidoreductases / genetics*,  metabolism*
Plant Proteins / biosynthesis
Proteome / metabolism
Purine Nucleotides / metabolism
Quaternary Ammonium Compounds / metabolism
Reactive Nitrogen Species / metabolism
Reactive Oxygen Species / metabolism
Reg. No./Substance:
0/Amino Acids; 0/Free Radical Scavengers; 0/Nitrates; 0/Nitrites; 0/Plant Proteins; 0/Proteome; 0/Purine Nucleotides; 0/Quaternary Ammonium Compounds; 0/Reactive Nitrogen Species; 0/Reactive Oxygen Species; 7727-37-9/Nitrogen; EC 1.-/Oxidoreductases; EC 1.-/alternative oxidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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