Document Detail


Plasmolysis and cell wall deposition in wheat root hairs under osmotic stress.
MedLine Citation:
PMID:  19533299     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We analysed cell wall formation in rapidly growing root hairs of Triticum aestivum under reduced turgor pressure by application of iso- and hypertonic mannitol solutions. Our experimental series revealed an osmotic value of wheat root hairs of 150 mOsm. In higher concentrations (200-650 mOsm), exocytosis of wall material and its deposition, as well as callose synthesis, still occurred, but the elongation of root hairs was stopped. Even after strong plasmolysis when the protoplast retreated from the cell wall, deposits of wall components were observed. Labelling with DiOC(6)(3) and FM1-43 revealed numerous Hechtian strands that spanned the plasmolytic space. Interestingly, the Hechtian strands also led towards the very tip of the root hair suggesting strong anchoring sites that are readily incorporated into the new cell wall. Long-term treatments of over 24 h in mannitol solutions (150-450 mOsm) resulted in reduced growth and concentration-dependent shortening of root hairs. However, the formation of new root hairs does occur in all concentrations used. This reflects the extraordinary potential of wheat root cells to adapt to environmental stress situations.
Authors:
Michael Volgger; Ingeborg Lang; Miroslav Ovecka; Irene Lichtscheidl
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-06-17
Journal Detail:
Title:  Protoplasma     Volume:  243     ISSN:  1615-6102     ISO Abbreviation:  Protoplasma     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-06-25     Completed Date:  2010-11-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9806853     Medline TA:  Protoplasma     Country:  Austria    
Other Details:
Languages:  eng     Pagination:  51-62     Citation Subset:  IM    
Affiliation:
Cell Imaging and Ultrastructure Research, Faculty of Life Sciences, The University of Vienna, Althanstrasse 14, 1090 Vienna, Austria.
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MeSH Terms
Descriptor/Qualifier:
Cell Wall / metabolism,  physiology*,  ultrastructure*
Fluorescent Dyes / metabolism
Osmotic Pressure
Plant Roots / growth & development*,  metabolism,  ultrastructure*
Triticum / anatomy & histology*,  growth & development*,  metabolism
Chemical
Reg. No./Substance:
0/Fluorescent Dyes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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