Document Detail


Plasmodium yoelii: identification of rhoptry proteins using monoclonal antibodies.
MedLine Citation:
PMID:  9806867     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Thirteen monoclonal antibodies, obtained after immunization of mice with Plasmodium yoelii schizonts, were selected using immunofluorescence assay: they all presented typical fluorescence patterns of rhoptries. This antigen localization was confirmed by immunoelectron microscopy. The molecular weights of the recognized antigens are 68, 80, 105, 130 and 140 kDa as determined by immunoprecipitation and immunoblot under reducing and nonreducing conditions. These values are very similar to these of the low and high molecular weight complex components of Plasmodium falciparum. Furthermore, these antigens are soluble like P. falciparum rhoptry proteins. Interestingly, our monoclonal antibodies also reacted with two other Plasmodium species (Plasmodium berghei NKK173 strain and P. yoelii nigeriensis 798 VK strain), giving sometimes more complex labeling with apical, membranous, nuclear, or/and cytoplasmic localizations. Finally, none of the monoclonal antibodies stained the rhoptries of P. falciparum FCCE-1/Niger strain.
Authors:
R Hienne; G Ricard; T Fusaï; H Fujioka; B Pradines; M Aikawa; J C Doury
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Experimental parasitology     Volume:  90     ISSN:  0014-4894     ISO Abbreviation:  Exp. Parasitol.     Publication Date:  1998 Nov 
Date Detail:
Created Date:  1998-12-01     Completed Date:  1998-12-01     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370713     Medline TA:  Exp Parasitol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  230-5     Citation Subset:  IM    
Copyright Information:
Copyright 1998 Academic Press.
Affiliation:
Institut de Médecine Tropicale du Service de Santé des Armées, Unité de Parasitologie, Parc du Pharo, Marseille Armées, 13998, France.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Monoclonal / immunology*
Antibodies, Protozoan / immunology
Antibody Specificity
Antigens, Protozoan / analysis*,  chemistry,  immunology
Blotting, Western
Female
Fluorescent Antibody Technique, Indirect
Humans
Hybridomas
Mice
Microscopy, Immunoelectron
Molecular Weight
Plasmodium yoelii / immunology*
Precipitin Tests
Protozoan Proteins / analysis*,  chemistry,  immunology
Solubility
Grant Support
ID/Acronym/Agency:
AI-35827/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antibodies, Protozoan; 0/Antigens, Protozoan; 0/Protozoan Proteins; 0/rhoptry associated protein, Plasmodium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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