Document Detail


Plasmodium falciparum glycosylphosphatidylinositol toxin interacts with the membrane of non-parasitized red blood cells: a putative mechanism contributing to malaria anemia.
MedLine Citation:
PMID:  18657459     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Following exposure to synthetic Plasmodium falciparum glycosylphosphatidylinositol (P.f.-GPI), red blood cells (RBCs) reacted with antibodies in the serum of a patient with severe acute P. falciparum malaria. Carbohydrate microarray analysis of the patient's serum confirmed the presence of both, IgM and IgG antibodies against P.f.-GPI. The antibodies failed to bind to RBCs when P.f.-GPI lacking the lipid portion was applied. Addition of the detergent Triton X-100 during preincubation with P.f.-GPI resulted in increased recognition. Recognition of P.f.-GPI was dependent on the concentrations of synthetic P.f.-GPI, the serum and the numbers of RBCs. IgM antibodies dominated P.f.-GPI-sensitized RBCs recognition. Recognition by IgM antibodies proved highest during the 1st week of acute malaria and decreased during the following 2 weeks as assessed by flow cytometry and carbohydrate microarray analysis. These results strongly support the notion that released P.f.-GPI can insert into non-parasitized RBC membranes and results in recognition by circulating anti-GPI antibodies and possibly subsequent elimination. This process may contribute to malaria-associated anemia.
Authors:
Norbert W Brattig; Katharina Kowalsky; Xinyu Liu; Gerd D Burchard; Faustin Kamena; Peter H Seeberger
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-05-14
Journal Detail:
Title:  Microbes and infection / Institut Pasteur     Volume:  10     ISSN:  1286-4579     ISO Abbreviation:  Microbes Infect.     Publication Date:  2008 Jul 
Date Detail:
Created Date:  2008-08-11     Completed Date:  2008-10-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100883508     Medline TA:  Microbes Infect     Country:  France    
Other Details:
Languages:  eng     Pagination:  885-91     Citation Subset:  IM    
Affiliation:
Section Tropical Medicine, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany. nbrattig@bni-hamburg.de
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MeSH Terms
Descriptor/Qualifier:
Anemia*
Animals
Antibodies, Protozoan / blood,  immunology
Cell Membrane / drug effects*
Erythrocytes / drug effects*
Glycosylphosphatidylinositols / toxicity*
Humans
Immunoglobulin M / blood,  immunology
Malaria / pathology
Middle Aged
Plasmodium falciparum / pathogenicity*
Time Factors
Virulence Factors / toxicity*
Chemical
Reg. No./Substance:
0/Antibodies, Protozoan; 0/Glycosylphosphatidylinositols; 0/Immunoglobulin M; 0/Virulence Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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