| Plasmodium falciparum: evaluation of lactate dehydrogenase in monitoring therapeutic responses to standard antimalarial drugs in Nigeria. | |
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MedLine Citation:
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PMID: 9371095 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The correlation of P. falciparum lactate dehydrogenase (pLDH) activities and patent infections was evaluated for monitoring therapeutic responses and drug resistance in 70 patients with microscopically confirmed P. falciparum malaria in Nigeria. Each patient was treated with standard dosages of artemether (53 patients), chloroquine (7 patients), sulfadoxine-pyrimethamine (6 patients), or halofantrine (4 patients). Response of infection to treatment was monitored by microscopic examination of thick and thin blood smears, clinical symptoms, and levels of pLDH activities in blood products. pLDH activity was determined using an antibody capture technique and 3-acetyl pyridine adenine dinucleotide developed to enhance sensitivity of the enzyme detection. All patients treated with artemether were cured while 5 patients treated with chloroquine, 1 treated with sulfadoxine-pyrimethamine, and 2 treated with halofantrine suffered recrudescent infections after treatment. pLDH activity was detected in blood products obtained from patients with patent or recrudescent infections determined by microscopy and clinical symptoms. Levels of pLDH activities in whole blood and packed cells from the patients correlated with qualitative detection of parasites in blood smears and in patients with high gametocyte counts. Gametocyte counts in the patients after treatment ranged from 40 gametocytes/microliter of blood to 4923 gametocytes/microliter of blood. There is a consistent relationship between patent infection and pLDH activities that could easily be determined in whole blood and packed cells from the patients. Further development of the procedure will enhance its valuable application in clinical management of drug-resistant malaria in the endemic areas. |
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Authors:
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A M Oduola; G O Omitowoju; A Sowunmi; M T Makler; C O Falade; D E Kyle; F A Fehintola; O A Ogundahunsi; R C Piper; B G Schuster; W K Milhous |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Experimental parasitology Volume: 87 ISSN: 0014-4894 ISO Abbreviation: Exp. Parasitol. Publication Date: 1997 Nov |
Date Detail:
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Created Date: 1997-12-23 Completed Date: 1997-12-23 Revised Date: 2009-08-18 |
Medline Journal Info:
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Nlm Unique ID: 0370713 Medline TA: Exp Parasitol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 283-9 Citation Subset: IM |
Affiliation:
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Department of Pharmacology and Therapeutics, College of Medicine, University of Ibadan, Nigeria. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Adult Antimalarials / therapeutic use* Artemisinins* Child Child, Preschool Chloroquine / therapeutic use Drug Combinations Drug Resistance Female Humans Infant L-Lactate Dehydrogenase / analysis* Malaria, Falciparum / blood, drug therapy*, enzymology, epidemiology Male Monitoring, Physiologic Nigeria / epidemiology Phenanthrenes / therapeutic use Pyrimethamine / therapeutic use Sesquiterpenes / therapeutic use Sulfadoxine / therapeutic use Treatment Outcome |
| Chemical | |
Reg. No./Substance:
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0/Antimalarials; 0/Artemisinins; 0/Drug Combinations; 0/Phenanthrenes; 0/Sesquiterpenes; 0/artemether; 2447-57-6/Sulfadoxine; 36167-63-2/halofantrine; 37338-39-9/sulfadoxine-pyrimethamine; 54-05-7/Chloroquine; 58-14-0/Pyrimethamine; EC 1.1.1.27/L-Lactate Dehydrogenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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