| Plasmodium falciparum: continuous cultivation of erythrocyte stages in plasma-free culture medium. | |
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MedLine Citation:
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PMID: 6363117 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Continuous in vitro cultivation of the malaria parasite, Plasmodium falciparum, was performed in plasma-free medium. The medium used was standard RPMI 1640 supplemented with adenosine, unsaturated C-18 fatty acids, and fatty acid-free bovine serum albumin. The medium was changed daily and the cultures were diluted with washed erythrocytes twice weekly. Growth was routinely maintained for 1 month at which time the experiments were usually terminated. Although the overall growth rates were consistently lower than in control cultures with plasma, continuous growth occurred in the absence of plasma in cultures containing cis-vaccenic, oleic, and linoleic acids. |
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Authors:
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G P Willet; C J Canfield |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Experimental parasitology Volume: 57 ISSN: 0014-4894 ISO Abbreviation: Exp. Parasitol. Publication Date: 1984 Feb |
Date Detail:
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Created Date: 1984-03-14 Completed Date: 1984-03-14 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0370713 Medline TA: Exp Parasitol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 76-80 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Culture Media / analysis* Erythrocytes / parasitology* Fatty Acids / pharmacology* Humans Kinetics Linoleic Acids / pharmacology Oleic Acid Oleic Acids / pharmacology Plasmodium falciparum / growth & development* |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 0/Fatty Acids; 0/Linoleic Acids; 0/Oleic Acids; 112-80-1/Oleic Acid; 506-17-2/cis-vaccenic acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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