| Plasmodium falciparum: nitric oxide modulates heme speciation in isolated food vacuoles. | |
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MedLine Citation:
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PMID: 20493843 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Nitric oxide (NO) and NO-derived reactive nitrogen species (RNS) are present in the food vacuole (FV) of Plasmodium falciparum trophozoites. The product of PFL1555w, a putative cytochrome b(5), localizes in the FV membrane, similar to what was previously observed for the product of PF13_0353, a putative cytochrome b(5) reductase. These two gene products may contribute to NO generation by denitrification chemistry from nitrate and/or nitrite present in the erythrocyte cytosol. The possible coordination of NO to heme species present in the food vacuole was probed by resonance Raman spectroscopy. The spectroscopic data revealed that in situ generated NO interacts with heme inside the intact FVs to form ferrous heme nitrosyl complexes that influence intra-vacuolar heme solubility. The formation of heme nitrosyl complexes within the FV is a previously unrecognized factor that could affect the equilibrium between soluble and crystallized heme within the FV in vivo. |
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Authors:
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Graciela Ostera; Fuyuki Tokumasu; Clarissa Teixeira; Nicolas Collin; Juliana Sa; Jennifer Hume; Sanjai Kumar; Jose Ribeiro; Gudrun S Lukat-Rodgers; Kenton R Rodgers |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2010-05-21 |
Journal Detail:
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Title: Experimental parasitology Volume: 127 ISSN: 1090-2449 ISO Abbreviation: Exp. Parasitol. Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2011-01-03 Completed Date: 2011-02-09 Revised Date: 2013-04-26 |
Medline Journal Info:
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Nlm Unique ID: 0370713 Medline TA: Exp Parasitol Country: United States |
Other Details:
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Languages: eng Pagination: 1-8 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier Inc. All rights reserved. |
Affiliation:
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Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD 20852, USA. osterag@georgetown.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Crystallization Erythrocytes / parasitology Heme / chemistry, metabolism* Humans Immune Sera / diagnostic use Immunoblotting Mice Microscopy, Fluorescence Nitric Oxide / metabolism* Plasmodium falciparum / genetics, metabolism*, ultrastructure Solubility Spectrum Analysis, Raman Vacuoles / chemistry, metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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AI072719/AI/NIAID NIH HHS; R15 AI072719/AI/NIAID NIH HHS; Z99 AI999999/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Immune Sera; 10102-43-9/Nitric Oxide; 14875-96-8/Heme |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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