Document Detail


Plasmodium falciparum: nitric oxide modulates heme speciation in isolated food vacuoles.
MedLine Citation:
PMID:  20493843     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Nitric oxide (NO) and NO-derived reactive nitrogen species (RNS) are present in the food vacuole (FV) of Plasmodium falciparum trophozoites. The product of PFL1555w, a putative cytochrome b(5), localizes in the FV membrane, similar to what was previously observed for the product of PF13_0353, a putative cytochrome b(5) reductase. These two gene products may contribute to NO generation by denitrification chemistry from nitrate and/or nitrite present in the erythrocyte cytosol. The possible coordination of NO to heme species present in the food vacuole was probed by resonance Raman spectroscopy. The spectroscopic data revealed that in situ generated NO interacts with heme inside the intact FVs to form ferrous heme nitrosyl complexes that influence intra-vacuolar heme solubility. The formation of heme nitrosyl complexes within the FV is a previously unrecognized factor that could affect the equilibrium between soluble and crystallized heme within the FV in vivo.
Authors:
Graciela Ostera; Fuyuki Tokumasu; Clarissa Teixeira; Nicolas Collin; Juliana Sa; Jennifer Hume; Sanjai Kumar; Jose Ribeiro; Gudrun S Lukat-Rodgers; Kenton R Rodgers
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2010-05-21
Journal Detail:
Title:  Experimental parasitology     Volume:  127     ISSN:  1090-2449     ISO Abbreviation:  Exp. Parasitol.     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2011-01-03     Completed Date:  2011-02-09     Revised Date:  2013-05-29    
Medline Journal Info:
Nlm Unique ID:  0370713     Medline TA:  Exp Parasitol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1-8     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Inc. All rights reserved.
Affiliation:
Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD 20852, USA. osterag@georgetown.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Crystallization
Erythrocytes / parasitology
Heme / chemistry,  metabolism*
Humans
Immune Sera / diagnostic use
Immunoblotting
Mice
Microscopy, Fluorescence
Nitric Oxide / metabolism*
Plasmodium falciparum / genetics,  metabolism*,  ultrastructure
Solubility
Spectrum Analysis, Raman
Vacuoles / chemistry,  metabolism*
Grant Support
ID/Acronym/Agency:
AI072719/AI/NIAID NIH HHS; R15 AI072719/AI/NIAID NIH HHS; Z99 AI999999/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Immune Sera; 10102-43-9/Nitric Oxide; 14875-96-8/Heme
Comments/Corrections

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