Document Detail


Plasmodium coatneyi in the rhesus monkey (Macaca mulatta) as a model of malaria in pregnancy.
MedLine Citation:
PMID:  9715932     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Pregnant women with Plasmodium falciparum infection are at increased risk for complications such as anemia and cerebral malaria. In addition, the infants of these women suffer intrauterine growth retardation (IUGR), low birth weight (LBW), congenital infection, and high infant mortality. Although much has been learned from studies of malaria during human pregnancy, progress has been limited by the lack of a suitable animal model. Nonhuman primates are of particular interest because, other than the armadillo, they are the only animals with a discoidal, villous, hemochorial placenta like that of humans. We have established a model of malaria during human pregnancy by inoculating pregnant rhesus monkeys (Macaca mulatta) with Plasmodium coatneyi (a sequestering parasite) during the first trimester. In our initial experiment, four monkeys were inoculated with a fresh inoculum containing 10(8) viable parasites from an infected donor monkey. All four monkeys became parasitemic seven days postinoculation (PI) and three monkeys aborted 7-10 days PI coincident with high peak parasitemias (41,088-374,325 parasites/mm3). Although abortion is one of the outcomes observed in Plasmodium-infected women, the intent of this study was to examine the effects of Plasmodium infection throughout gestation. Since the rapid onset of high parasitemia may have been responsible for the abortions, a decision was made to reduce the size of the effective inoculum. Six additional pregnant monkeys were inoculated with a frozen isolate taken from the same donor containing 10(6) parasites. These six animals became parasitemic by 14 days PI and, along with monkey E412, carried their infants to term. These seven infants weighed significantly less at term than the infants of uninfected mothers (P = 0.0355). Symmetrical IUGR was detected by ultrasound in one fetus with an LBW of 334 g. Another LBW infant (300 g) had asymmetrical growth retardation, which has been associated with uteroplacental insufficiency and was consistent with the lower placental weights found in infected dams compared with controls (P = 0.0455). The infant with symmetric IUGR died at five days of age, while the other is alive but congenitally infected. The IUGR, LBW, congenital infection, postnatal infant mortality, and early abortions observed in these animals suggest that P. coatneyi in pregnant rhesus monkeys is a valid model of malaria in human pregnancy. This model should provide the opportunity to study questions about malaria in pregnancy that have been difficult to study in humans.
Authors:
B B Davison; F B Cogswell; G B Baskin; K P Falkenstein; E W Henson; A F Tarantal; D J Krogstad
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of tropical medicine and hygiene     Volume:  59     ISSN:  0002-9637     ISO Abbreviation:  Am. J. Trop. Med. Hyg.     Publication Date:  1998 Aug 
Date Detail:
Created Date:  1998-09-02     Completed Date:  1998-09-02     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370507     Medline TA:  Am J Trop Med Hyg     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  189-201     Citation Subset:  AIM; IM    
Affiliation:
Department of Pathology, Tulane Regional Primate Research Center, and Tulane School of Public Health and Tropical Medicine, Tulane University Medical Center, New Orleans, Louisiana 70433, USA.
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MeSH Terms
Descriptor/Qualifier:
Abortion, Veterinary / parasitology
Anemia / parasitology
Animals
Animals, Newborn
Disease Models, Animal*
Female
Fetal Growth Retardation / parasitology
Humans
Macaca mulatta*
Malaria / complications,  etiology*,  physiopathology
Parasitemia / complications,  etiology*,  physiopathology
Placenta / pathology
Pregnancy
Pregnancy Complications, Hematologic / parasitology
Pregnancy Complications, Parasitic / etiology*,  physiopathology
Pregnancy Outcome
Grant Support
ID/Acronym/Agency:
1K01RR00075/RR/NCRR NIH HHS; 5P51-RR0016431/RR/NCRR NIH HHS

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