| Plasmodesmata transport of GFP alone or fused to potato virus X TGBp1 is diffusion driven. | |
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MedLine Citation:
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PMID: 18767215 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Plasmodesmata (Pd) provide a pathway for exchanging various macromolecules between neighboring plant cells. Researchers routinely characterize the mobility of the green-fluorescent protein (GFP) and GFP fusions through Pd by calculating the proportion of sites in bombarded leaves which show fluorescence in multiple cell clusters (% movement). Here, the Arrhenius equation was used to describe the temperature dependence of GFP and GFP-TGBpl (potato virus X triple gene block protein1) movement, using % movement values, and to calculate the activation energy for protein transport. The resulting low activation energy indicates GFP and GFP-TGBp1 movement are diffusion driven. Furthermore, GFP movement is inversely proportional to the leaf surface area of expanding leaves. The increase in leaf area results mainly from cell expansion during the sink-source transition. The increasing cell size results in lower Pd density, which decreases the probability that a GFP attains an open Pd by diffusion. The decline in GFP movement as leaf area expands indicates that, in addition to GFP diffusion through Pd, attaining an open Pd by undirected diffusion might be limiting for Pd transport. In summary, this report provides a new quantitative method for studying Pd conductivity. |
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Authors:
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G Schönknecht; J E Brown; J Verchot-Lubicz |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Protoplasma Volume: 232 ISSN: 0033-183X ISO Abbreviation: Protoplasma Publication Date: 2008 |
Date Detail:
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Created Date: 2008-09-03 Completed Date: 2008-09-17 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9806853 Medline TA: Protoplasma Country: Austria |
Other Details:
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Languages: eng Pagination: 143-52 Citation Subset: IM |
Affiliation:
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Botany Department, Oklahoma State University, Stillwater, Oklahoma 74078, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Biolistics Biological Transport Diffusion Green Fluorescent Proteins / metabolism* Plant Leaves / cytology, metabolism Plasmids / genetics Plasmodesmata / metabolism* Potexvirus / metabolism* Recombinant Fusion Proteins / metabolism* Temperature Tobacco / cytology, metabolism Viral Proteins / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Recombinant Fusion Proteins; 0/Viral Proteins; 147336-22-9/Green Fluorescent Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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