Document Detail

Plasminogen activator inhibitor type 1 promotes fibrosarcoma cell migration by modifying cellular attachment to vitronectin via alpha(v)beta(5) integrin.
MedLine Citation:
PMID:  16052409     Owner:  NLM     Status:  MEDLINE    
Both urokinase plasminogen activator (u-PA) and plasminogen activator inhibitor type 1 (PAI-1) are associated with a poor prognosis in cancer patients. We demonstrate that PAI-1 inhibits human fibrosarcoma cell (HT-1080) adhesion to vitronectin (Vn) via alpha (v)beta (5) integrin, and stimulates cell migration from Vn toward collagen type IV (Col). The cells attached more strongly to Vn and Col than to fibronectin (Fn), whereas PAI-1 interfered with cell attachment to Vn only. An integrin antagonist, RGD peptide, and anti-alpha (v)beta (5) integrin antibodies, which similarly inhibited cell attachment to Vn, also stimulated cell migration from Vn toward Col. u-PA did not modify cell attachment directly, but reversed the PAI-1-mediated inhibitory effect on cell adhesion to Vn, and its stimulatory effect on cell migration from Vn toward Col. Thus HT-1080 cell migration appears to be modified by u-PA and PAI-1, altering cell adhesion to Vn via alpha (v)beta (5) integrin. This may be related to their tumor-promoting effect.
Tsuyoshi Takahashi; Kazuya Suzuki; Hayato Ihara; Hideo Mogami; Teruhisa Kazui; Tetsumei Urano
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Seminars in thrombosis and hemostasis     Volume:  31     ISSN:  0094-6176     ISO Abbreviation:  Semin. Thromb. Hemost.     Publication Date:  2005 Jun 
Date Detail:
Created Date:  2005-07-29     Completed Date:  2005-10-18     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0431155     Medline TA:  Semin Thromb Hemost     Country:  United States    
Other Details:
Languages:  eng     Pagination:  356-63     Citation Subset:  IM    
First Department of Surgery, Hamamatsu University School of Medicine, Hamamatsu, Shizuoka.
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MeSH Terms
Cell Adhesion
Cell Line, Tumor
Cell Movement*
Collagen Type IV / metabolism
Fibronectins / metabolism
Fibrosarcoma / pathology*
Integrins / metabolism*
Plasminogen Activator Inhibitor 1 / physiology*
Receptors, Vitronectin / metabolism*
Urokinase-Type Plasminogen Activator / physiology
Vitronectin / metabolism*
Reg. No./Substance:
0/Collagen Type IV; 0/Fibronectins; 0/Integrins; 0/Plasminogen Activator Inhibitor 1; 0/Receptors, Vitronectin; 0/Vitronectin; 0/integrin alphaVbeta5; EC Plasminogen Activator

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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