| Plasminogen activator inhibitor-1 mRNA is expressed in platelets and megakaryocytes and the megakaryoblastic cell line CHRF-288. | |
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MedLine Citation:
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PMID: 8485117 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Plasminogen activator inhibitor-1 (PAI-1) is present in the platelet alpha-granule and is released on platelet activation. Platelet PAI-1 could either be synthesized by the megakaryocyte or taken up from the plasma. In this report we confirm the presence of PAI-1 protein in human megakaryocytes by Western blot analysis and show its synthesis in guinea pig megakaryocytes by metabolic labeling. We document the presence of PAI-1 mRNA in human platelets and show a 3-kb mRNA species on Northern blot analysis of guinea pig megakaryocytes. Neither untreated CHRF-288 cells, a megakaryoblastic cell line, nor human erythroleukemia (HEL) cells expressed PAI-1 mRNA. Phorbol ester (phorbol 12-myristate 13-acetate, 160 nM) treatment of CHRF-288 and HEL cells for 4 days induced PAI-1 mRNA expression in CHRF-288 cells but not in HEL cells. These studies show that PAI-1 is synthesized by megakaryocytes. Megakaryocytes most likely determine the PAI-1 content of platelets and thereby establish the antifibrinolytic potential of the platelet. |
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Authors:
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B A Konkle; P K Schick; X He; R J Liu; E M Mazur |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Arteriosclerosis and thrombosis : a journal of vascular biology / American Heart Association Volume: 13 ISSN: 1049-8834 ISO Abbreviation: Arterioscler. Thromb. Publication Date: 1993 May |
Date Detail:
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Created Date: 1993-06-10 Completed Date: 1993-06-10 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 9101388 Medline TA: Arterioscler Thromb Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 669-74 Citation Subset: IM |
Affiliation:
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Cardeza Foundation for Hematologic Research, Jefferson Medical College, Thomas Jefferson University, Philadelphia, PA 19107. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blood Platelets / metabolism* Blotting, Northern Blotting, Western Guinea Pigs Humans Leukemia, Erythroblastic, Acute / metabolism* Megakaryocytes / metabolism* Plasminogen Activator Inhibitor 1 / genetics* Polymerase Chain Reaction Precipitin Tests RNA, Messenger / metabolism* Tetradecanoylphorbol Acetate / pharmacology Tumor Cells, Cultured |
| Grant Support | |
ID/Acronym/Agency:
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HL-25455/HL/NHLBI NIH HHS; HL-44956/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Plasminogen Activator Inhibitor 1; 0/RNA, Messenger; 16561-29-8/Tetradecanoylphorbol Acetate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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