| Plasmin-mediated proteolysis of casein in bovine milk. | |
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MedLine Citation:
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PMID: 156365 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Plasminogen was found to be present in bovine milk by crossreactivity between rabbit antiserum to plasminogen and casein prepared from milk by acid precipitation. This result was further supported by recovery of intact 125I-labeled plasminogen from rabbit milk after its intravenous injection. Freshly isolated whole bovine casein was observed to undergo slow autoproteolysis at 37 degrees C. Polyacrylamide gel electrophoresis revealed gradual disappearance of major caseins accompanied by appearance and increase in intensity of numerous electrophoretic bands. This autoproteolysis was inhibited by low concentrations of epsilon-aminocaproic acid (0.1 mM) and diisopropyl fluorophosphate (1 mM); catalytic amounts of urokinase accelerated the process. Autoproteolysis of isolated bovine beta-casein was shown by both urea and sodium dodecyl sulfate gel electrophoresis to result in formation of gamma 1- and gamma 2-caseins. Similar electrophoretic bands were formed when beta-casein was degraded by plasmin prepared from bovine blood serum. These results support the hypothesis that bovine plasmin occurs in milk and is identical to alkaline milk protease. |
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Authors:
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W N Eigel; C J Hofmann; B A Chibber; J M Tomich; T W Keenan; E T Mertz |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 76 ISSN: 0027-8424 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 1979 May |
Date Detail:
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Created Date: 1979-08-29 Completed Date: 1979-08-29 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 2244-8 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Caseins / metabolism* Cattle Female Fibrinolysin / antagonists & inhibitors, metabolism* Hydrolysis Milk / enzymology* Molecular Weight Plasminogen / metabolism Protease Inhibitors / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Caseins; 0/Protease Inhibitors; 9001-91-6/Plasminogen; EC 3.4.21.7/Fibrinolysin |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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