| Plasmids carrying blaCTX-M-1 and qnr genes in Escherichia coli isolates from an equine clinic and a horseback riding centre. | |
| | |
MedLine Citation:
|
PMID: 21393204 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Objectives The aim of this study was to determine the occurrence of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli at an equine clinic and a horseback riding centre, and to discuss the impact of antimicrobial treatment on resistance selection. Methods Faeces from horses, environmental smears and flies were sampled at both the clinic and riding centre. Staff at the equine clinic were also examined. The samples were cultivated on MacConkey agar with cefotaxime (2 mg/L) to isolate ESBL-producing E. coli. The presence of bla and qnr genes was tested by PCR, and transferability was determined by conjugation. Replicon typing and restriction analysis of plasmids harbouring ESBL and qnr genes were performed. Results E. coli with the bla(CTX-M-1) gene were isolated from horses, staff, environmental smears and flies at the two sites. E. coli isolates from the equine clinic harboured an IncHI1 conjugative 235-285 kb plasmid containing bla(CTX-M-1), catA1, strA, sul2 and tet(B) genes. Some of these were positive for qnrS1 and/or qnrB19, and were located on 40 or 45 kb IncN or IncX1 conjugative plasmids. The gene bla(CTX-M-1) in isolates from the riding centre was carried by IncN (30 kb) and IncI1 (85 kb) conjugative plasmids. Horizontal gene transfer seems to be involved in disseminating E. coli with ESBL and qnr genes at the clinic and riding centre. Conclusions The study illustrates that ESBL-producing E. coli, as well as plasmids carrying ESBL genes of clinical interest, can be easily transferred among horses, humans and flies living in close contact. |
| | |
Authors:
|
Monika Dolejska; Eva Duskova; Jana Rybarikova; Dagmar Janoszowska; Eva Roubalova; Katerina Dibdakova; Gabriela Maceckova; Ludmila Kohoutova; Ivan Literak; Jiri Smola; Alois Cizek |
Related Documents
:
|
21477784 - A rice mutant displaying a heterochronically elongated internode carries a 100 kb dele... 21535354 - Identification of serine/threonine kinase and nucleotide-binding site-leucine-rich repe... 17943314 - Comparative analysis of essential genes and nonessential genes in escherichia coli k12. 17387144 - Genomic regulatory blocks encompass multiple neighboring genes and maintain conserved s... 8407834 - An escherichia coli gene in search of a function: phenotypic effects of the gene recent... 10698334 - Fhl-1/reconectin and factor h: two human complement regulators which are encoded by the... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-1-25 |
Journal Detail:
|
Title: The Journal of antimicrobial chemotherapy Volume: - ISSN: 1460-2091 ISO Abbreviation: - Publication Date: 2011 Jan |
Date Detail:
|
Created Date: 2011-3-11 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 7513617 Medline TA: J Antimicrob Chemother Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho 1-3, 612 42 Brno, Czech Republic. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Impetigo in a population over 8.5 years: incidence, fusidic acid resistance and molecular characteri...
Next Document: Clinical experience with daptomycin in Europe: the first 2.5 years.