| Plasmid stability of recombinant Pseudomonas sp. B13 FR1 pFRC20P in continuous culture. | |
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MedLine Citation:
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PMID: 10099179 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Plasmid stability of recombinant Pseudomonas sp. B13 FR1 pFRC20P, a strain capable of mineralizing 3- and 4-chlorobenzoate and 4-methylbenzoate, was investigated in continuous culture. The hybrid cosmid pFRC20P enables the strain to mineralize 4-methylbenzoate. Rapid plasmid loss was observed under nonselective conditions using 3-chlorobenzoate as the substrate. Plasmid stability decreased with increasing dilution rate. Despite the growth advantage of the generated plasmid free cells a total depletion of plasmid bearing cells was not observed. After approximately 50 generations the fraction of plasmid bearing cells reached a constant level of 10%, which was stably maintained during the next 25 generations. Cells from this stage were used to inoculate a new culture that resulted in a stable level of 50% plasmid bearing cells. By a temporary substrate change to selective conditions (4-methylbenzoate), this level could be further increased to 70%. Literature models on plasmid stability could not be applied to describe the experimental data. Therefore, a new but unstructured model was developed to describe the experimental results. The model is based on the existence of three subpopulations: a plasmid free one, an original plasmid bearing one with a growth disadvantage compared to plasmid free cells, and a second plasmid bearing subpopulation with increased stability that is generated from the original one and has a growth rate comparable to the plasmid free cells. |
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Authors:
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C Hempel; R W Erb; W Deckwer; V Hecht |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biotechnology and bioengineering Volume: 57 ISSN: 0006-3592 ISO Abbreviation: Biotechnol. Bioeng. Publication Date: 1998 Jan |
Date Detail:
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Created Date: 1999-05-07 Completed Date: 1999-05-07 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7502021 Medline TA: Biotechnol Bioeng Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 62-70 Citation Subset: IM |
Copyright Information:
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Copyright 1998 John Wiley & Sons, Inc. |
Affiliation:
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Biochemical Engineering Division, GBF-Gesellschaft für Biotechnologische Forschung mbH, Mascheroder Weg 1, 38124 Braunschweig, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Benzoic Acids
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metabolism Biodegradation, Environmental Bioreactors Biotechnology Chlorobenzoates / metabolism Models, Biological Plasmids / genetics* Pseudomonas / genetics*, metabolism Recombination, Genetic |
| Chemical | |
Reg. No./Substance:
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0/Benzoic Acids; 0/Chlorobenzoates; 535-80-8/3-chlorobenzoic acid; 74-11-3/4-chlorobenzoic acid; 99-94-5/4-toluic acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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