| Plasmid, serotypic, and enterotoxin analysis of Bacillus cereus in an outbreak setting. | |
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MedLine Citation:
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PMID: 3139708 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Bacillus cereus is a recognized agent of food-borne disease. In this report we describe an outbreak of B. cereus gastroenteritis associated with consumption of beef stew among patients and staff at a Rhode Island nursing home. The beef had been improperly stored after preparation. The predominant symptoms of the illness were cramps and diarrhea; it lasted an average of 16 h. No deaths occurred. The organism was recovered from 10 of 23 stools collected from ill patients and 1 of 21 stools collected from controls (P = 0.0044, Fisher's two-tailed exact test). All isolates had the same biotype and serotype, newly designated H.26; all elaborated the diarrheal B. cereus enterotoxin when tested in rabbits by the vascular permeability reaction; and all had identical plasmid profiles, which differed from those of B. cereus strains selected randomly from other outbreaks. Plasmid analysis may prove to be a useful new tool in investigating outbreaks of B. cereus food poisoning. |
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Authors:
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B A DeBuono; J Brondum; J M Kramer; R J Gilbert; S M Opal |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of clinical microbiology Volume: 26 ISSN: 0095-1137 ISO Abbreviation: J. Clin. Microbiol. Publication Date: 1988 Aug |
Date Detail:
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Created Date: 1988-11-23 Completed Date: 1988-11-23 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 7505564 Medline TA: J Clin Microbiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1571-4 Citation Subset: IM |
Affiliation:
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Brown University Program in Medicine, Providence, Rhode Island 02912. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aged Aged, 80 and over Animals Bacillus cereus / classification*, genetics Bacterial Infections / epidemiology, microbiology* Bacterial Typing Techniques DNA, Bacterial / analysis Disease Outbreaks* Enterotoxins / analysis Foodborne Diseases / epidemiology, microbiology* Gastroenteritis / epidemiology, microbiology* Homes for the Aged Humans Meat Nursing Homes Plasmids Questionnaires Rabbits Serotyping |
| Chemical | |
Reg. No./Substance:
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0/DNA, Bacterial; 0/Enterotoxins |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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