Document Detail

Plasmid, serotypic, and enterotoxin analysis of Bacillus cereus in an outbreak setting.
MedLine Citation:
PMID:  3139708     Owner:  NLM     Status:  MEDLINE    
Bacillus cereus is a recognized agent of food-borne disease. In this report we describe an outbreak of B. cereus gastroenteritis associated with consumption of beef stew among patients and staff at a Rhode Island nursing home. The beef had been improperly stored after preparation. The predominant symptoms of the illness were cramps and diarrhea; it lasted an average of 16 h. No deaths occurred. The organism was recovered from 10 of 23 stools collected from ill patients and 1 of 21 stools collected from controls (P = 0.0044, Fisher's two-tailed exact test). All isolates had the same biotype and serotype, newly designated H.26; all elaborated the diarrheal B. cereus enterotoxin when tested in rabbits by the vascular permeability reaction; and all had identical plasmid profiles, which differed from those of B. cereus strains selected randomly from other outbreaks. Plasmid analysis may prove to be a useful new tool in investigating outbreaks of B. cereus food poisoning.
B A DeBuono; J Brondum; J M Kramer; R J Gilbert; S M Opal
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of clinical microbiology     Volume:  26     ISSN:  0095-1137     ISO Abbreviation:  J. Clin. Microbiol.     Publication Date:  1988 Aug 
Date Detail:
Created Date:  1988-11-23     Completed Date:  1988-11-23     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  7505564     Medline TA:  J Clin Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1571-4     Citation Subset:  IM    
Brown University Program in Medicine, Providence, Rhode Island 02912.
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MeSH Terms
Aged, 80 and over
Bacillus cereus / classification*,  genetics
Bacterial Infections / epidemiology,  microbiology*
Bacterial Typing Techniques
DNA, Bacterial / analysis
Disease Outbreaks*
Enterotoxins / analysis
Foodborne Diseases / epidemiology,  microbiology*
Gastroenteritis / epidemiology,  microbiology*
Homes for the Aged
Nursing Homes
Reg. No./Substance:
0/DNA, Bacterial; 0/Enterotoxins

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