Document Detail

Plasmid screening at high colony density.
MedLine Citation:
PMID:  6997135     Owner:  NLM     Status:  MEDLINE    
A procedure is described for screening bacterial colonies containing recombinant plasmids by nucleic acid hybridization at high density, i.e., at 100 000 colonies per 150 mm diameter plate. Small colonies are established on nitrocellulose filters from which they can be faithfully replicated to additional filters. Chloramphenicol amplification may be carried out in situ before screening. The filters may be kept frozen for long-term storage of colonies which may be further replicated after thawing.
D Hanahan; M Meselson
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Gene     Volume:  10     ISSN:  0378-1119     ISO Abbreviation:  Gene     Publication Date:  1980 Jun 
Date Detail:
Created Date:  1980-11-20     Completed Date:  1980-11-20     Revised Date:  2006-05-01    
Medline Journal Info:
Nlm Unique ID:  7706761     Medline TA:  Gene     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  63-7     Citation Subset:  IM    
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MeSH Terms
Cloning, Molecular / methods*
DNA, Recombinant
Escherichia coli / genetics
Nucleic Acid Hybridization
Reg. No./Substance:
0/DNA, Recombinant

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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