Document Detail

Plasmid modifications in a tick-borne pathogen, Borrelia burgdorferi, cocultured with tick cells.
MedLine Citation:
PMID:  8269098     Owner:  NLM     Status:  MEDLINE    
We describe an in vitro system that will facilitate molecular analysis of the association between Lyme disease spirochetes and vector cells. We cocultured Borrelia burgdorferi continuously with two tick cell lines, RAE25 (from Rhipicephalus appendiculatus) and IDE8 (from Ixodes scapularis). A clone isolated after twenty-two passages with RAE25 cells had lost the largest (49 kb) plasmid, and probes containing information normally encoded on it, including genes for two surface proteins, hybridized to smaller plasmids. Spirochetes maintained with IDE 8 cells showed a new 43 kb plasmid that hybridized to a probe made from the 49 kb plasmid. After reisolation from hamsters, these spirochetes carried a large plasmid (100 kb) that hybridized with the 49 kb plasmid. These changes may illustrate a plasticity that enables B. burgdorferi to adapt to different environments.
U G Munderloh; Y J Park; J M Dioh; A M Fallon; T J Kurtti
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Insect molecular biology     Volume:  1     ISSN:  0962-1075     ISO Abbreviation:  Insect Mol. Biol.     Publication Date:  1993  
Date Detail:
Created Date:  1994-01-28     Completed Date:  1994-01-28     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9303579     Medline TA:  Insect Mol Biol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  195-203     Citation Subset:  IM    
Department of Entomology, University of Minnesota, St Paul 55108.
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MeSH Terms
Arachnid Vectors / cytology,  microbiology*
Borrelia burgdorferi Group / genetics*
Cell Line
Plasmids / genetics*
Ticks / cytology,  microbiology*
Grant Support

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