| Plasmid maintenance and physiology of a genetically engineered Escherichia coli strain during continuous L-carnitine production. | |
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MedLine Citation:
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PMID: 17581701 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effect of immobilization on cell physiology and how this determines cell metabolic performance is an important concern for developing bioprocess. This is particularly true for genetically modified microorganisms and their genetic stability. For this reason the stability and physiological state of plasmid-bearing E. coli cells were ascertained by flow cytometry. Differences in the cellular DNA and protein content (15-20%) permit discrimination of control and plasmid-bearing cells, as well as adaptation to continuous cultivation conditions in both freely suspended and immobilized states to be monitored. Moreover, the observed metabolic burden due to maintenance and over-expression of plasmid-coded genetic material and slow cell growth in poorly-viable immobilized cells were found to be the main factors contributing to strain stabilization. |
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Authors:
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Vicente Bernal; María González-Veracruz; Manuel Cánovas; José L Iborra |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-06-21 |
Journal Detail:
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Title: Biotechnology letters Volume: 29 ISSN: 0141-5492 ISO Abbreviation: Biotechnol. Lett. Publication Date: 2007 Oct |
Date Detail:
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Created Date: 2007-08-22 Completed Date: 2007-12-13 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8008051 Medline TA: Biotechnol Lett Country: Netherlands |
Other Details:
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Languages: eng Pagination: 1549-56 Citation Subset: IM |
Affiliation:
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Departamento de Bioquímica y Biología Molecular B e Inmunología, Facultad de Química, Universidad de Murcia, P.O. Box 4021, 30100, Murcia, Spain. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacterial Adhesion
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physiology Bacterial Proteins / metabolism Biomass Carnitine / biosynthesis*, metabolism Carrageenan / chemistry, metabolism DNA, Bacterial / metabolism Escherichia coli / genetics, metabolism*, physiology Flow Cytometry Microspheres Models, Biological Plasmids / genetics* RNA, Bacterial / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/DNA, Bacterial; 0/RNA, Bacterial; 541-15-1/Carnitine; 9000-07-1/Carrageenan |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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