Document Detail


Plasmid maintenance and physiology of a genetically engineered Escherichia coli strain during continuous L-carnitine production.
MedLine Citation:
PMID:  17581701     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effect of immobilization on cell physiology and how this determines cell metabolic performance is an important concern for developing bioprocess. This is particularly true for genetically modified microorganisms and their genetic stability. For this reason the stability and physiological state of plasmid-bearing E. coli cells were ascertained by flow cytometry. Differences in the cellular DNA and protein content (15-20%) permit discrimination of control and plasmid-bearing cells, as well as adaptation to continuous cultivation conditions in both freely suspended and immobilized states to be monitored. Moreover, the observed metabolic burden due to maintenance and over-expression of plasmid-coded genetic material and slow cell growth in poorly-viable immobilized cells were found to be the main factors contributing to strain stabilization.
Authors:
Vicente Bernal; María González-Veracruz; Manuel Cánovas; José L Iborra
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-06-21
Journal Detail:
Title:  Biotechnology letters     Volume:  29     ISSN:  0141-5492     ISO Abbreviation:  Biotechnol. Lett.     Publication Date:  2007 Oct 
Date Detail:
Created Date:  2007-08-22     Completed Date:  2007-12-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8008051     Medline TA:  Biotechnol Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1549-56     Citation Subset:  IM    
Affiliation:
Departamento de Bioquímica y Biología Molecular B e Inmunología, Facultad de Química, Universidad de Murcia, P.O. Box 4021, 30100, Murcia, Spain.
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MeSH Terms
Descriptor/Qualifier:
Bacterial Adhesion / physiology
Bacterial Proteins / metabolism
Biomass
Carnitine / biosynthesis*,  metabolism
Carrageenan / chemistry,  metabolism
DNA, Bacterial / metabolism
Escherichia coli / genetics,  metabolism*,  physiology
Flow Cytometry
Microspheres
Models, Biological
Plasmids / genetics*
RNA, Bacterial / metabolism
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/DNA, Bacterial; 0/RNA, Bacterial; 541-15-1/Carnitine; 9000-07-1/Carrageenan

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