Document Detail

Plasmid R1 is present as clusters in the cells of Escherichia coli.
MedLine Citation:
PMID:  10783298     Owner:  NLM     Status:  MEDLINE    
Fluorescence microscopy was used to determine the location(s) of the replication origin of plasmid R1 in exponentially growing cells of Escherichia coli. The number of oriR1 foci per cell was smaller than the number of R1 copies per cell and was found to be the same for a copA mutant of R1 and for the wild-type plasmid. The intensities of individual foci were stronger for the cop mutant than for the wild type. We interpreted these results to imply that the plasmid DNA molecules were localized in small groups/clusters, a result that seems contrary to the earlier observations that plasmid R1 replicates randomly and segregates as a single-copy unit. The implications for the quantitative behavior of plasmid R1 in stability, incompatibility tests, replication, and partition experiments are discussed.
T Weitao; S Dasgupta; K Nordström
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Plasmid     Volume:  43     ISSN:  0147-619X     ISO Abbreviation:  Plasmid     Publication Date:  2000 May 
Date Detail:
Created Date:  2000-07-19     Completed Date:  2000-07-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7802221     Medline TA:  Plasmid     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  200-4     Citation Subset:  IM    
Copyright Information:
Copyright 2000 Academic Press.
Department of Cell and Molecular Biology, Uppsala University, Biomedical Center, Uppsala, S-751 24, Sweden.
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MeSH Terms
Escherichia coli / genetics*
Gene Dosage
Multigene Family
Plasmids / genetics*
Replication Origin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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