Document Detail


Plasmalemma-bound nucleoside diphosphatase as a cytochemical marker of central nervous system (CNS) mesodermal cells.
MedLine Citation:
PMID:  6281329     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Nucleoside diphosphatase (NDPase) activity, using inosine-5'-diphospbate (IDP) as a substrate, was studied by light and electron microscope cytochemistry in brain and spinal cord of normal mouse. After cytochemical incubation for the enzyme, neurons, astroglial and oligodendroglial cells contained reaction product in the cisternae of endoplasmic reticulum (ER) and/or in the trans elements of the Golgi apparatus. In contrast, reaction product for NDPase was concentrated on the outer surface of the plasmalemma of microglial cells, endothelial cells of microvessels, pericytes, and smooth muscle cells of the arteriole wall. An occasional microglial cell contained sparse reaction product in the flattened saccules of the Golgi apparatus. The pattern of enzyme distribution for these cell types was the same for all parts of the nervous system examined in this study. These data suggest that in cells of ectodermal origin, NDPase is intracellular in location, whereas in cells of mesodermal origin, this enzyme is bound to the plasmalemma. The usefulness of this cytochemical reaction for NDPase as a marker of microglial and other cells in brain pathology is discussed.
Authors:
A W Vorbrodt; H M Wisniewski
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society     Volume:  30     ISSN:  0022-1554     ISO Abbreviation:  J. Histochem. Cytochem.     Publication Date:  1982 May 
Date Detail:
Created Date:  1982-07-19     Completed Date:  1982-07-19     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9815334     Medline TA:  J Histochem Cytochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  418-24     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Acid Anhydride Hydrolases*
Animals
Astrocytes / enzymology
Brain / enzymology*,  ultrastructure
Endoplasmic Reticulum / ultrastructure
Golgi Apparatus / ultrastructure
Mice
Mice, Inbred Strains
Microscopy, Electron
Neurons / enzymology
Oligodendroglia / enzymology
Phosphoric Monoester Hydrolases / metabolism*
Spinal Cord / enzymology*,  ultrastructure
Subcellular Fractions / enzymology,  ultrastructure
Grant Support
ID/Acronym/Agency:
17271-01//PHS HHS
Chemical
Reg. No./Substance:
EC 3.1.3.-/Phosphoric Monoester Hydrolases; EC 3.6.-/Acid Anhydride Hydrolases; EC 3.6.1.6/nucleoside-diphosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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