| Plasma and urine profiles of Delta9-tetrahydrocannabinol and its metabolites 11-hydroxy-Delta9-tetrahydrocannabinol and 11-nor-9-carboxy-Delta9-tetrahydrocannabinol after cannabis smoking by male volunteers to estimate recent consumption by athletes. | |
| | |
MedLine Citation:
|
PMID: 20112012 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Since 2004, cannabis has been prohibited by the World Anti-Doping Agency for all sports competitions. In the years since then, about half of all positive doping cases in Switzerland have been related to cannabis consumption. In doping urine analysis, the target analyte is 11-nor-9-carboxy-Delta(9)-tetrahydrocannabinol (THC-COOH), the cutoff being 15 ng/mL. However, the wide urinary detection window of the long-term metabolite of Delta(9)-tetrahydrocannabinol (THC) does not allow a conclusion to be drawn regarding the time of consumption or the impact on the physical performance. The purpose of the present study on light cannabis smokers was to evaluate target analytes with shorter urinary excretion times. Twelve male volunteers smoked a cannabis cigarette standardized to 70 mg THC per cigarette. Plasma and urine were collected up to 8 h and 11 days, respectively. Total THC, 11-hydroxy-Delta(9)-tetrahydrocannabinol (THC-OH), and THC-COOH were determined after hydrolysis followed by solid-phase extraction and gas chromatography/mass spectrometry. The limits of quantitation were 0.1-1.0 ng/mL. Eight puffs delivered a mean THC dose of 45 mg. Plasma levels of total THC, THC-OH, and THC-COOH were measured in the ranges 0.2-59.1, 0.1-3.9, and 0.4-16.4 ng/mL, respectively. Peak concentrations were observed at 5, 5-20, and 20-180 min. Urine levels were measured in the ranges 0.1-1.3, 0.1-14.4, and 0.5-38.2 ng/mL, peaking at 2, 2, and 6-24 h, respectively. The times of the last detectable levels were 2-8, 6-96, and 48-120 h. Besides high to very high THC-COOH levels (245 +/- 1,111 ng/mL), THC (3 +/- 8 ng/mL) and THC-OH (51 +/- 246 ng/mL) were found in 65 and 98% of cannabis-positive athletes' urine samples, respectively. In conclusion, in addition to THC-COOH, the pharmacologically active THC and THC-OH should be used as target analytes for doping urine analysis. In the case of light cannabis use, this may allow the estimation of more recent consumption, probably influencing performance during competitions. However, it is not possible to discriminate the intention of cannabis use, i.e., for recreational or doping purposes. Additionally, pharmacokinetic data of female volunteers are needed to interpret cannabis-positive doping cases of female athletes. |
| | |
Authors:
|
Rudolf Brenneisen; Pascale Meyer; Haithem Chtioui; Martial Saugy; Matthias Kamber |
Publication Detail:
|
Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't Date: 2010-01-30 |
Journal Detail:
|
Title: Analytical and bioanalytical chemistry Volume: 396 ISSN: 1618-2650 ISO Abbreviation: Anal Bioanal Chem Publication Date: 2010 Apr |
Date Detail:
|
Created Date: 2010-03-19 Completed Date: 2010-06-29 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101134327 Medline TA: Anal Bioanal Chem Country: Germany |
Other Details:
|
Languages: eng Pagination: 2493-502 Citation Subset: IM |
Affiliation:
|
Department of Clinical Research, University of Berne, Murtenstr. 35, 3010 Berne, Switzerland. rudolf.brenneisen@dkf.unibe.ch |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adult Cannabis / metabolism* Doping in Sports / prevention & control* Gas Chromatography-Mass Spectrometry / methods* Humans Male Smoking / blood, urine Street Drugs / blood*, urine* Substance Abuse Detection / methods* Tetrahydrocannabinol / blood*, urine* |
| Chemical | |
Reg. No./Substance:
|
0/Street Drugs; 1972-08-3/Tetrahydrocannabinol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Requirements for screening and confirmatory methods for the detection and quantification of marine b...
Next Document: Epithelioid hemangioendothelioma of the liver with metastatic coeliac lymph nodes in an 11-year-old ...