Document Detail

Plasma protein binding of polyphenols from maritime pine bark extract (USP).
MedLine Citation:
PMID:  20732774     Owner:  NLM     Status:  MEDLINE    
Maritime pine bark extract is monographed in the United States Pharmacopeia (USP) as a dietary supplement. As knowledge about active principles - protein interactions contribute to insights into pharmacokinetic and pharmacodynamic properties we elucidated the plasma protein binding of various constituents and metabolites of this extract. We chose high performance affinity chromatography for fast characterization of the analytes' binding extent to human serum albumin. For selected maritime pine bark compounds that were previously detected in human plasma samples we additionally employed ultrafiltration. The flavonoids catechin and taxifolin revealed highest plasma protein binding of close to 100%, followed by procyanidin B1 and the cinnamic acid derivates ferulic acid (73.5 ± 0.12%), caffeic acid (66.0 ± 0.23%) and p-cumaric acid (65.4 ± 4.84%). Lower protein binding was observed for the benzoic acid derivates vanillic acid (56.3 ± 1.16%), p-hydroxy benzoic acid (35.3 ± 10.9%), gallic acid (31.6 ± 0.56%) and protocatechuic acid (20.7 ± 0.09%). Lowest protein binding was measured for the maritime pine bark metabolites δ-(3,4-dihydroxy-phenyl)-γ-valerolactone (34.9 ± 1.28%) and δ-(3-methoxy-4-hydroxy-phenyl)-γ-valerolactone (26.4 ± 0.03%). For all but one compound the results of both methods revealed excellent correlation. Thus, we provide new data as a basis for a more comprehensive understanding of the bioactivity of a complex plant extract.
Max Kurlbaum; Petra Högger
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Publication Detail:
Type:  Journal Article     Date:  2010-08-06
Journal Detail:
Title:  Journal of pharmaceutical and biomedical analysis     Volume:  54     ISSN:  1873-264X     ISO Abbreviation:  J Pharm Biomed Anal     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2010-09-27     Completed Date:  2011-01-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8309336     Medline TA:  J Pharm Biomed Anal     Country:  England    
Other Details:
Languages:  eng     Pagination:  127-32     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier B.V. All rights reserved.
Universität Würzburg, Institut für Pharmazie und Lebensmittelchemie, Am Hubland, 97074 Würzburg, Germany.
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MeSH Terms
Blood Proteins / chemistry*
Catechin / chemistry
Chromatography, High Pressure Liquid / methods
Flavonoids / analysis,  chemistry*
Hydrogen Bonding
Models, Chemical
Phenols / analysis,  chemistry*
Pinus / metabolism*
Plant Extracts / analysis*
Protein Binding
Quercetin / analogs & derivatives,  chemistry
Regression Analysis
Reg. No./Substance:
0/Blood Proteins; 0/Buffers; 0/Flavonoids; 0/Phenols; 0/Plant Extracts; 0/polyphenols; 117-39-5/Quercetin; 154-23-4/Catechin; 480-18-2/taxifolin

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