| Plasma and myocardial visfatin expression changes are associated with therapeutic hypothermia protection during murine hemorrhagic shock/resuscitation. | |
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MedLine Citation:
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PMID: 20347206 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIM: Cytokine production during hemorrhagic shock (HS) could affect cardiac function during the hours after resuscitation. Visfatin is a recently described protein that functions both as a proinflammatory plasma cytokine and an intracellular enzyme within the nicotinamide adenine dinucleotide (NAD(+)) salvage pathway. We developed a mouse model of HS to study the effect of therapeutic hypothermia (TH) on hemodynamic outcomes and associated plasma and tissue visfatin content. METHODS: Mice were bled and maintained at a mean arterial pressure (MAP) of 35 mmHg. After 30 min, animals (n=52) were randomized to normothermia (NT, 37+/-0.5 degrees C) or TH (33+/-0.5 degrees C) followed by rewarming at 60 min following resuscitation. After 90 min of HS (S90), mice were resuscitated and monitored for 180 min (R180). Visfatin, interleukin 6 (IL-6), keratinocyte-derived chemokine (KC), tumor necrosis factor-alpha (TNF-alpha), and myoglobin were measured by ELISA. RESULTS: Compared to NT, TH animals exhibited improved R180 survival (23/26 [88.5%] vs. 13/26 [50%]; p=0.001). Plasma visfatin, IL-6, KC, and TNF-alpha increased by S90 in both groups (p<0.05). TH attenuated S90 plasma visfatin and, after rewarming, decreased R180 plasma IL-6, KC, and myoglobin (p<0.05) relative to NT. Heart and gut KC increased at S90 while IL-6 increases were delayed until R180 (p<0.05). NT produced sustained elevations of myocardial KC but decreased visfatin by R180, effects abrogated by TH (p<0.05). CONCLUSIONS: In a mouse model of HS, TH improves hemodynamics and alters plasma and tissue proinflammatory cytokines including the novel cytokine visfatin. TH modulation of cytokines may attenuate cardiac dysfunction following HS. |
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Authors:
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David G Beiser; Huashan Wang; Jing Li; Xu Wang; Violeta Yordanova; Anshuman Das; Tamara Mirzapoiazova; Joe G N Garcia; Susan A Stern; Terry L Vanden Hoek |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. Date: 2010-03-26 |
Journal Detail:
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Title: Resuscitation Volume: 81 ISSN: 1873-1570 ISO Abbreviation: Resuscitation Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-05-17 Completed Date: 2010-09-07 Revised Date: 2011-07-28 |
Medline Journal Info:
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Nlm Unique ID: 0332173 Medline TA: Resuscitation Country: Ireland |
Other Details:
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Languages: eng Pagination: 742-8 Citation Subset: IM |
Copyright Information:
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Copyright 2010 Elsevier Ireland Ltd. All rights reserved. |
Affiliation:
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Section of Emergency Medicine, University of Chicago, Chicago, IL 60637, USA. dbeiser@uchicago.edu |
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cardiopulmonary Resuscitation* Chemokines / blood Enzyme-Linked Immunosorbent Assay Female Hemodynamics Hypothermia, Induced* Ileum / metabolism Interleukin-6 / blood Mice Mice, Inbred C57BL Myocardium / metabolism* Myoglobin / blood Nicotinamide Phosphoribosyltransferase / blood, metabolism* Shock, Hemorrhagic / physiopathology, therapy* Survival Rate Time Factors Tumor Necrosis Factor-alpha / blood |
| Grant Support | |
ID/Acronym/Agency:
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K08 HL091184-01A1/HL/NHLBI NIH HHS; K08 HL091184-02/HL/NHLBI NIH HHS; K08 HL091184-03/HL/NHLBI NIH HHS; K08HL091184/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Chemokines; 0/Interleukin-6; 0/Myoglobin; 0/Tumor Necrosis Factor-alpha; 147037-79-4/keratinocyte-derived chemokines; EC 2.4.2.12/Nicotinamide Phosphoribosyltransferase |
| Comments/Corrections | |
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