Document Detail


Plasma miR-208 as a biomarker of myocardial injury.
MedLine Citation:
PMID:  19696117     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: MicroRNAs (miRNAs) are endogenous small RNAs of 21-25 nucleotides that can pair with sites in 3' untranslated regions in mRNAs of protein-coding genes to downregulate their expression. Recently, circulating miRNAs have been reported as promising biomarkers for various pathologic conditions. We assessed the hypothesis that miRNAs may leak into the circulating blood from injured cells and thereby serve as biomarkers for identifying the injured cell type. METHODS: We used isoproterenol-induced myocardial injury in rats as a model and miRNA array analyses to identify candidate miRNAs specifically produced in the ventricles of the heart. Individual miRNA concentrations were measured by real-time reverse-transcription PCR. Plasma cardiac troponin I (cTnI) concentrations were measured with an ELISA. RESULTS: Array analyses revealed miR-208 to be produced exclusively in the heart, and we selected this miRNA as a possible biomarker of myocardial injury. Plasma concentrations of miR-208 increased significantly (P < 0.0001) after isoproterenol-induced myocardial injury and showed a similar time course to the concentration of cTnI, a classic biomarker of myocardial injury. CONCLUSIONS: The plasma concentration of miR-208 may be a useful indicator of myocardial injury. Our results suggest that profiling of circulating miRNAs may help identify promising biomarkers of various pathologic conditions.
Authors:
Xu Ji; Rie Takahashi; Yumiko Hiura; Go Hirokawa; Yasue Fukushima; Naoharu Iwai
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-08-20
Journal Detail:
Title:  Clinical chemistry     Volume:  55     ISSN:  1530-8561     ISO Abbreviation:  Clin. Chem.     Publication Date:  2009 Nov 
Date Detail:
Created Date:  2009-10-29     Completed Date:  2009-11-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9421549     Medline TA:  Clin Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1944-9     Citation Subset:  IM    
Affiliation:
Department of Epidemiology, Research Institute, National Cardiovascular Center, Osaka 565-8565, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Aspartate Aminotransferases / blood
Cardiomegaly / genetics
Heart Injuries / chemically induced,  diagnosis*
Isoproterenol
Kidney / blood supply,  pathology
Male
MicroRNAs / analysis*,  blood,  diagnostic use*
Myocardium / metabolism,  pathology*
Rats
Rats, Sprague-Dawley
Reverse Transcriptase Polymerase Chain Reaction
Chemical
Reg. No./Substance:
0/MicroRNAs; 7683-59-2/Isoproterenol; EC 2.6.1.1/Aspartate Aminotransferases

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