Document Detail


Plasma membrane injury induced by nonyl gallate in Saccharomyces cerevisiae.
MedLine Citation:
PMID:  12010543     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIMS: The aim was to investigate the antifungal actions of nonyl gallate against Saccharomyces cerevisiae ATCC 7754. METHODS AND RESULTS: The maximum potency of both the growth inhibitory and the fungicidal effect against the yeast strain was found in nonyl gallate among n-alkyl gallates tested. Nonyl gallate induced ROS generation dose-dependently in growing cells. This ester rapidly killed yeast cells even when cell division was restricted by cycloheximide. This ester inhibited glucose-induced medium acidification and promoted the efflux of intracellular potassium ions in a nongrowing condition. Moreover, nonyl gallate induced a leakage of calcein from artificially prepared liposomes to a greater extent than dodecyl gallate did. CONCLUSIONS: These results suggested nonyl gallate injured plasma membrane of S. cerevisiae, resulting in its exhibition of fungicidal effect accompanying with a leakage of intracellular materials from the cells. SIGNIFICANCE AND IMPACT OF THE STUDY: Our study reveals new knowledge on the antifungal actions of nonyl gallate against S. cerevisiae. When nonyl gallate is applied as a food preservative, the level of its addition to foods may be reduced because of its potent antifungal activity compared with weak acids including sorbic acid and benzoic acid.
Authors:
K Fujita; I Kubo
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of applied microbiology     Volume:  92     ISSN:  1364-5072     ISO Abbreviation:  J. Appl. Microbiol.     Publication Date:  2002  
Date Detail:
Created Date:  2002-05-15     Completed Date:  2002-07-26     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9706280     Medline TA:  J Appl Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1035-42     Citation Subset:  IM    
Affiliation:
Department of Environmental Science, Policy and Management, University of California, Berkeley, California, USA.
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MeSH Terms
Descriptor/Qualifier:
Acids / metabolism
Adsorption
Antifungal Agents / pharmacology*
Antioxidants / pharmacology
Cell Membrane / drug effects*
Culture Media / metabolism
Cycloheximide / pharmacology
Esters / pharmacology
Gallic Acid / analogs & derivatives*,  pharmacology*
Glucose / pharmacology
Potassium / metabolism
Reactive Oxygen Species / metabolism
Saccharomyces cerevisiae / drug effects*,  metabolism
Chemical
Reg. No./Substance:
0/Acids; 0/Antifungal Agents; 0/Antioxidants; 0/Culture Media; 0/Esters; 0/Reactive Oxygen Species; 0/nonyl gallate; 1166-52-5/lauryl gallate; 149-91-7/Gallic Acid; 50-99-7/Glucose; 66-81-9/Cycloheximide; 7440-09-7/Potassium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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