Document Detail


Plasma membrane aquaporin AqpZ protein is essential for glucose metabolism during photomixotrophic growth of Synechocystis sp. PCC 6803.
MedLine Citation:
PMID:  21558269     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The genome of Synechocystis PCC 6803 contains a single gene encoding an aquaporin, aqpZ. The AqpZ protein functioned as a water-permeable channel in the plasma membrane. However, the physiological importance of AqpZ in Synechocystis remains unclear. We found that growth in glucose-containing medium inhibited proper division of ΔaqpZ cells and led to cell death. Deletion of a gene encoding a glucose transporter in the ΔaqpZ background alleviated the glucose-mediated growth inhibition of the ΔaqpZ cells. The ΔaqpZ cells swelled more than the wild type after the addition of glucose, suggesting an increase in cytosolic osmolarity. This was accompanied by a down-regulation of the pentose phosphate pathway and concurrent glycogen accumulation. Metabolite profiling by GC/TOF-MS of wild-type and ΔaqpZ cells revealed a relative decrease of intermediates of the tricarboxylic acid cycle and certain amino acids in the mutant. The changed levels of metabolites may have been the cause for the observed decrease in growth rate of the ΔaqpZ cells along with decreased PSII activity at pH values ranging from 7.5 to 8.5. A mutant in sll1961, encoding a putative transcription factor, and a Δhik31 mutant, lacking a putative glucose-sensing kinase, both exhibited higher glucose sensitivity than the ΔaqpZ cells. Examination of protein expression indicated that sll1961 functioned as a positive regulator of aqpZ gene expression but not as the only regulator. Overall, the ΔaqpZ cells showed defects in macronutrient metabolism, pH homeostasis, and cell division under photomixotrophic conditions, consistent with an essential role of AqpZ in glucose metabolism.
Authors:
Masaro Akai; Kiyoshi Onai; Miyako Kusano; Mayuko Sato; Henning Redestig; Kiminori Toyooka; Megumi Morishita; Hiroshi Miyake; Akihiro Hazama; Vanessa Checchetto; Ildikò Szabò; Ken Matsuoka; Kazuki Saito; Masato Yasui; Masahiro Ishiura; Nobuyuki Uozumi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-05-10
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  286     ISSN:  1083-351X     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2011 Jul 
Date Detail:
Created Date:  2011-07-15     Completed Date:  2012-01-24     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  25224-35     Citation Subset:  IM    
Affiliation:
Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University Aobayama 6-6-07, Sendai 980-8579, Japan.
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MeSH Terms
Descriptor/Qualifier:
Aquaporins / genetics,  metabolism*
Bacterial Proteins / genetics,  metabolism*
Cell Membrane / genetics,  metabolism*
Cytosol / metabolism
Gene Deletion
Glucose / metabolism*
Glucose Transport Proteins, Facilitative / genetics,  metabolism
Osmolar Concentration
Pentose Phosphate Pathway / physiology
Synechocystis / genetics,  metabolism*
Chemical
Reg. No./Substance:
0/Aquaporins; 0/Bacterial Proteins; 0/Glucose Transport Proteins, Facilitative; 50-99-7/Glucose
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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