Document Detail

Plasma, liver and kidney folate and plasma homocysteine concentrations are poor response variables at very low dietary folate intakes, in a folate depletion/repletion rat model.
MedLine Citation:
PMID:  11820095     Owner:  NLM     Status:  MEDLINE    
Folate depletion/repletion rat models are popular protocols for assessing the bioavailability of folate. Much of the early work carried out on folate bioavailability concentrated on foods naturally high in folate. However, foods low in folate often contribute significantly to folate intake because of their high consumption in the general population. Therefore, the assessment of the bioavailability of foods low in folate is essential to properly estimate folate intake. The present study investigated plasma, liver and kidney folate and plasma homocysteine concentrations as appropriate response variables for measuring folate bioavailability in the rat at very low dietary folate intakes. One hundred and one weanling male rats (Wistar strain) were fed a folate-deficient diet containing 1% succinyl sulfathiazole for 28 d. Following depletion, six rats were randomly assigned to each of 16 repletion diets containing folic acid, fortified white bread, unfortified wholemeal bread or unfortified rye bread calculated to provide 6.25, 12.5, 18.75 and 25 micrograms folate/kg of each diet. After a further 28 d, plasma, liver and kidney folate concentrations were determined by microbiological assay. Plasma homocysteine was measured by HPLC as a functional indicator of folate status. Only a weak correlation was found between the response variables measured and dietary folate intake, indicating that this folate depletion/repletion rat model is not suitable for testing the response of rats fed diets containing very low levels of folate.
K O'Leary; P J Sheehy
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  International journal of food sciences and nutrition     Volume:  53     ISSN:  0963-7486     ISO Abbreviation:  Int J Food Sci Nutr     Publication Date:  2002 Jan 
Date Detail:
Created Date:  2002-01-31     Completed Date:  2002-02-07     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  9432922     Medline TA:  Int J Food Sci Nutr     Country:  England    
Other Details:
Languages:  eng     Pagination:  35-42     Citation Subset:  IM    
Department of Food Science and Technology, University College, Cork, Republic of Ireland.
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MeSH Terms
Biological Availability
Biological Markers / analysis,  blood
Folic Acid / administration & dosage*,  analysis,  metabolism
Folic Acid Deficiency / diet therapy*,  metabolism
Food, Fortified
Homocysteine / blood
Kidney / chemistry
Liver / chemistry
Models, Animal*
Random Allocation
Rats, Wistar
Reg. No./Substance:
0/Biological Markers; 454-28-4/Homocysteine; 59-30-3/Folic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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