Document Detail


Plasma levels of matrix metalloproteinase-9 in chronic urticaria patients correlate with disease severity and C-reactive protein but not with circulating histamine-releasing factors.
MedLine Citation:
PMID:  20214668     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Matrix metalloproteinase-9 (MMP-9) is an endopeptidase produced by many inflammatory cells that has been found in increased amounts in plasma from patients with chronic urticaria (CU). OBJECTIVE: To evaluate plasma levels of MMP-9 and its tissue inhibitor of metalloproteinase-1 (TIMP-1) in CU patients in relation with disease severity, C-reactive protein (CRP) and circulating histamine-releasing factors. METHODS: Fifty-two consecutive CU patients were included in the study and disease activity was graded from 0 to 3. Plasma MMP-9, TIMP-1 and CRP levels were measured by enzyme immunoassays. Circulating histamine-releasing factors were assessed using in vivo (autologous serum skin test) and in vitro (basophil histamine release) tests. Seven CU patients were studied both during active disease and during remission. Thirty healthy subjects were used as normal controls. RESULTS: Plasma levels of MMP-9, TIMP-1 and CRP were significantly higher in CU patients than in healthy controls (P=0.0001, 0.003 and 0.005, respectively) and a trend towards a higher MMP-9/TIMP-1 molar ratio was found (P=0.051). A significant correlation was found between plasma MMP-9 levels and urticaria severity score (r=0.48, P<0.0001). CRP levels correlated with MMP-9 levels (r=0.37, P=0.008) and CU severity score (r=0.52, P=0.0001), but not with TIMP-1 (r=0.13) concentrations. MMP-9, TIMP-1 and CRP plasma levels and MMP-9/TIMP-1 molar ratio did not differ in patients either with or without an evidence of circulating histamine-releasing factors. Seven patients evaluated during remission showed a significant reduction of MMP-9 and CRP plasma levels. CONCLUSION: Plasma levels of MMP-9 and its inhibitor TIMP-1 are increased in CU patients. MMP-9 levels are associated with disease severity and CRP levels, but not with skin reactivity to autologous serum and with circulating histamine-releasing factors. These findings suggest that in CU there is an ongoing inflammatory process independent of the presence of circulating histamine-releasing factors.
Authors:
A Tedeschi; R Asero; M Lorini; A V Marzano; M Cugno
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Publication Detail:
Type:  Journal Article     Date:  2010-03-04
Journal Detail:
Title:  Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology     Volume:  40     ISSN:  1365-2222     ISO Abbreviation:  Clin. Exp. Allergy     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-06-18     Completed Date:  2010-10-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8906443     Medline TA:  Clin Exp Allergy     Country:  England    
Other Details:
Languages:  eng     Pagination:  875-81     Citation Subset:  IM    
Affiliation:
Unità Operativa di Allergologia e Immunologia Clinica, Ospedale Maggiore Policlinico, Mangiagalli e Regina Elena, Fondazione Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS), Milano, Italy. albited@alice.it
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MeSH Terms
Descriptor/Qualifier:
Adult
C-Reactive Protein / metabolism*
Chronic Disease
Enzyme-Linked Immunosorbent Assay
Female
Humans
Male
Matrix Metalloproteinase 9 / blood*
Severity of Illness Index
Skin Tests
Tissue Inhibitor of Metalloproteinase-1 / blood
Tumor Markers, Biological / blood
Urticaria / blood,  physiopathology*
Chemical
Reg. No./Substance:
0/Tissue Inhibitor of Metalloproteinase-1; 0/Tumor Markers, Biological; 0/translationally controlled tumor protein, p23; 9007-41-4/C-Reactive Protein; EC 3.4.24.35/Matrix Metalloproteinase 9
Comments/Corrections
Comment In:
Clin Exp Allergy. 2010 Jun;40(6):834-5   [PMID:  20214660 ]

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