Document Detail


Plasma endogenous enkephalin levels in early systemic sclerosis: clinical and laboratory associations.
MedLine Citation:
PMID:  20576209     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: Met- and leu-enkephalins are endogenous opioid neuropeptides with potent analgesic, vasoactive, immunomodulatory and anti-apoptotic properties. We hypothesised that clinical or immunological variables of early systemic sclerosis (SSc) might be correlated to plasma enkephalin levels.
METHODS: Plasma samples were collected at study entry of the Genetics versus Environment in Scleroderma Outcomes Study (GENISOS) cohort (early SSc, n=116). Plasma met-enkephalin and leu-enkephalin levels (microg/ml) were measured by high performance liquid chromatography (HPLC) and correlated to clinical and laboratory parameters in the GENISOS database. Statistical analyses were performed by nonparametric Wilcoxon rank sum tests and Pearson correlation coefficients.
RESULTS: Significantly lower plasma met-enkephalin levels were associated with anti-topoisomerase-I seropositivity (6+8.3 vs. 14.9+22.8 microg/ml, p=0.02). Plasma leu-enkephalin levels were significantly higher in SSc patients with digital pulp loss (95.6+130 vs. 64.9+101 microg/ml, p=0.02). Lower mean plasma met-enkephalin levels and inversely higher leu-enkephalin levels were noted in SSc patients with Raynaud's phenomena (p=NS).
CONCLUSION: The associations of plasma enkephalin levels to immunologic or clinical pathologies may underscore their vasogenic or fibrogenic significance and potential as therapeutic targets in early SSc.
Authors:
Terry A McNearney; K A Sluka; C Ahn; J D Reveille; Michael Fischbach; Maureen D Mayes
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2010-06-10
Journal Detail:
Title:  Clinical and experimental rheumatology     Volume:  28     ISSN:  0392-856X     ISO Abbreviation:  Clin. Exp. Rheumatol.     Publication Date:    2010 Mar-Apr
Date Detail:
Created Date:  2010-08-31     Completed Date:  2010-09-16     Revised Date:  2011-10-12    
Medline Journal Info:
Nlm Unique ID:  8308521     Medline TA:  Clin Exp Rheumatol     Country:  Italy    
Other Details:
Languages:  eng     Pagination:  S7-11     Citation Subset:  IM    
Affiliation:
Division of Rheumatology-UTMB, 301 University Blvd., Galveston, TX 77555-1165, USA. tmcnearn@utmb.edu
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MeSH Terms
Descriptor/Qualifier:
Autoantibodies / immunology
Chromatography, High Pressure Liquid
DNA Topoisomerases, Type I / immunology
Enkephalin, Leucine / blood*,  physiology
Enkephalin, Methionine / blood*,  physiology
Female
Humans
Male
Middle Aged
Neurotransmitter Agents / blood*,  physiology
Scleroderma, Diffuse / blood*,  immunology,  physiopathology
Scleroderma, Limited / blood*,  immunology,  physiopathology
Grant Support
ID/Acronym/Agency:
AR052316/AR/NIAMS NIH HHS; K0202201//PHS HHS; M01-RR00073/RR/NCRR NIH HHS; M01-RR01346/RR/NCRR NIH HHS; M01-RR02558/RR/NCRR NIH HHS; NS39734/NS/NINDS NIH HHS; P50 AR044888-06/AR/NIAMS NIH HHS; P50 AR054144-03/AR/NIAMS NIH HHS; P50AR054144/AR/NIAMS NIH HHS; P50AR44888/AR/NIAMS NIH HHS
Chemical
Reg. No./Substance:
0/Autoantibodies; 0/Neurotransmitter Agents; 58569-55-4/Enkephalin, Methionine; 58822-25-6/Enkephalin, Leucine; EC 5.99.1.2/DNA Topoisomerases, Type I

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