| Plasma amino acid quantitation using gas chromatography chemical ionization mass spectrometry and 13C amino acids as internal standards. | |
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MedLine Citation:
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PMID: 749957 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A specific and sensitive method for the quantitation of 16 alpha amino acids has been developed. The technique employed uses methane chemical ionization gas chromatography mass spectrometry of the carboxy-n-butyl, N-trifluoroacetyl amino acid derivatives. A commercial 13C amino acid mixture provided individual internal standards for 14 alpha amino acids. A computer controlled quadrupole mass spectrometer was used for selected ion monitoring of those ions characteristic of each N-trifluoroacetyl amino acid/13C amino acid pair. A BASIC computer program located peak maxima and background intensities in each selected ion recording. Standard curves for each amino acid/13C amino acid pair were utilized by the program to calculate the plasma concentration of each detected amino acid. The total instrumental analysis occupied 30 min with sample preparation and derivatization accounting for an additional 2 h. Based on the detection of known amounts of standard amino acids the method will quantitate at the 1-5 nanogram level of detection. |
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Authors:
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E E Kingston; A M Duffield |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biomedical mass spectrometry Volume: 5 ISSN: 0306-042X ISO Abbreviation: Biomed. Mass Spectrom. Publication Date: 1978 Nov |
Date Detail:
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Created Date: 1979-07-16 Completed Date: 1979-07-16 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0430246 Medline TA: Biomed Mass Spectrom Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 621-6 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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blood* Carbon Isotopes Chromatography, Gas Computers Humans Ions Mass Spectrometry / methods* Metabolism, Inborn Errors / blood |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Carbon Isotopes; 0/Ions |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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