Document Detail


Plasma GLP-2 levels and intestinal markers in the juvenile pig during intestinal adaptation: effects of different diet regimens.
MedLine Citation:
PMID:  15573929     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Adaptation of the residual small bowel following resection is dependent on luminal and humoral factors. We aimed to establish if circulating levels of glucagon-like peptide (GLP-2) change under different dietary regimens following resection and to determine if there is a relationship between plasma GLP-2 levels and markers of intestinal adaptation. Four-week-old piglets underwent a 75% proximal small bowel resection (n = 31) or transection (n = 14). Postoperatively they received either pig chow (n = 14), nonpolymeric (elemental) infant formula (n = 7), or polymeric infant formula alone (n = 8) or supplemented either with fiber (n = 6) or with bovine colostrum protein concentrate (CPC; n = 10) for 8 weeks until sacrifice. Plasma GLP-2 levels were measured at weeks 0, 2, 4, and 8 postoperatively. In addition, end-stage parameters were studied at week 8 including weight gain, ileal villus height, crypt depth, and disaccharidase levels. Plasma GLP-2 levels were higher in resected animals compared to transected animals fed the same diet. Plasma GLP-2 levels were significantly increased in the colostrum protein isolate-supplemented animals following resection compared to all other diet groups. The increase in plasma GLP-2 (pM) was greatest in the first 2 weeks postresection (week 0, 15.5; week 2, 30.9), followed by a plateau at weeks 2 to 4 and a decrease in GLP-2 levels from week 4 to week 8. At week 8, no relationships were found between the plasma GLP-2 levels and the measurements of weight gain, villus height, lactase, sucrase, maltase, crypt depth, or villus/crypt ratio. Plasma GLP-2 levels increase in the first weeks following massive small intestinal resection. The increase in plasma GLP-2 levels was enhanced by supplementation of the diet with CPC. The changes in GLP-2 levels observed in this study may suggest that GLP-2 plays a role in the adaptive response in the intestine following resection in this preclinical model.
Authors:
Monique C Paris; Peter J Fuller; Bendix Carstensen; Eva Nagy; Russell G Taylor; Magdy Sourial; Jens J Holst; Bolette Hartmann; Julie E Binesm
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Digestive diseases and sciences     Volume:  49     ISSN:  0163-2116     ISO Abbreviation:  Dig. Dis. Sci.     Publication Date:  2004 Oct 
Date Detail:
Created Date:  2004-12-02     Completed Date:  2004-12-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7902782     Medline TA:  Dig Dis Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1688-95     Citation Subset:  AIM; IM    
Affiliation:
Department of Gastroenterology and Clinical Nutrition, Royal Children's Hospital, Parkville, Victoria, Australia. monique.paris@mcri.edu.au
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MeSH Terms
Descriptor/Qualifier:
Adaptation, Physiological / physiology*
Animals
Diet*
Dietary Supplements
Disease Models, Animal
Energy Intake
Female
Glucagon-Like Peptide 2
Glucagon-Like Peptides
Intestine, Small / physiology*,  surgery
Peptides / blood*
Postoperative Period
Short Bowel Syndrome / blood,  physiopathology
Swine
Chemical
Reg. No./Substance:
0/Glucagon-Like Peptide 2; 0/Peptides; 62340-29-8/Glucagon-Like Peptides

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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