Document Detail


Plasma 21-deoxycortisol: comparison of a time-resolved fluoroimmunoassay using a biotinylated tracer with a radioimmunossay using (125)iodine.
MedLine Citation:
PMID:  10731638     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Plasma 21-deoxycortisol (21DF) is an excellent marker of 21-hydroxylase deficiency. Currently, it is the only marker able to detect heterozygous carriers with 21-hydroxylase deficiency after ACTH stimulation. We have already developed radioimmunoassays for 21DF using first tritiated, then 125I-21DF which had a ten-fold higher sensitivity. However, because the lifespan of 125I-21DF is short, the tracer needs to be reprepared every two months and this multiplies the risk of contamination by radioactive 125I vapours. We therefore developed a non-isotopic 21DF assay that uses a 21DF-biotin conjugate with a original bridge, a diaminopropyl arm, linking the steroid to biotin. The 21DF-biotin conjugate was measured by time-resolved fluorescence after adding streptavidin-europium to the microtitration wells. The analytical qualities of this assay were very similar to those of the radioimmunoassay using 125I-21DF as tracer. The results obtained by the two methods, in either normal subjects or patients with 21-hydroxylase deficiency, were virtually the same.
Authors:
J Fiet; A Boudi; F Giton; J M Villette; P Boudou; H Soliman; G Morineau; H Galons
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  The Journal of steroid biochemistry and molecular biology     Volume:  72     ISSN:  0960-0760     ISO Abbreviation:  J. Steroid Biochem. Mol. Biol.     Publication Date:    2000 Jan-Feb
Date Detail:
Created Date:  2000-05-08     Completed Date:  2000-05-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9015483     Medline TA:  J Steroid Biochem Mol Biol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  55-60     Citation Subset:  IM    
Affiliation:
Laboratoire d'Hormonologie, Hôpital St. Louis, 75010, Paris, France.
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MeSH Terms
Descriptor/Qualifier:
Adrenal Hyperplasia, Congenital*
Adult
Age of Onset
Biotinylation
Calibration
Cortodoxone / blood*,  immunology
Cross Reactions / immunology
Cross-Linking Reagents
Europium / metabolism
Female
Fluorescence
Fluoroimmunoassay / methods*
Humans
Immune Sera / immunology
Iodine Radioisotopes
Male
Menstrual Cycle
Radioimmunoassay / methods*
Reproducibility of Results
Sensitivity and Specificity
Streptavidin / metabolism
Time Factors
Chemical
Reg. No./Substance:
0/Cross-Linking Reagents; 0/Immune Sera; 0/Iodine Radioisotopes; 152-58-9/Cortodoxone; 641-77-0/21-deoxycortisol; 7440-53-1/Europium; 9013-20-1/Streptavidin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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