| Plaque morphology of Italian H7N1 LPAI isolates in MDCK cells and in primary cells of different avian species. | |
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MedLine Citation:
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PMID: 14575134 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The morphology of plaques induced by Italian, H7N1, low-pathogenic avian influenza (LPAI) viruses belonging to different lineages was investigated in primary chicken, turkey, Muscovy duck, and mallard duck kidney cells and in MDCK cells in the absence of trypsin. LPAI isolates were selected on the basis of the location in the phylogenetic tree: 977/V99 (located at the root, no additional glycosylation site [nAGS]), 2379N99 (AGS in position 123), and 3675/V99 (AGS in position 149). Different isolates did not induce plaques with a statistically significant different size in MDCK cells. However, in primary cells of different avian origin, the presence or absence of AGS significantly influenced plaque size. Generally speaking, 977N99 was the least efficient at plaquing in all cells, while 2379/V99 (AGS in position 123) plaqued more efficiently in turkey cells and 3675/V99 (AGS in position 149) in chicken cells. The presence of either AGS induced statistically significant larger plaques in cells of waterfowl origin. |
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Authors:
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G Cattoli; V Brasola; I Capua |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Avian diseases Volume: 47 ISSN: 0005-2086 ISO Abbreviation: Avian Dis. Publication Date: 2003 |
Date Detail:
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Created Date: 2003-10-24 Completed Date: 2003-12-10 Revised Date: 2005-11-22 |
Medline Journal Info:
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Nlm Unique ID: 0370617 Medline TA: Avian Dis Country: United States |
Other Details:
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Languages: eng Pagination: 1161-3 Citation Subset: IM |
Affiliation:
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National Reference Laboratory for Newcastle Disease and Avian Influenza, Istituto Zooprofilattico Sperimentale delle Venezie, Via Romea 14/A, 35020, Legnaro, (PD) Italy. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Line Dogs Glycosylation Hemagglutinins, Viral / chemistry*, physiology Influenza A virus / isolation & purification, pathogenicity, ultrastructure* Italy Plaque Assay / methods* Species Specificity |
| Chemical | |
Reg. No./Substance:
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0/Hemagglutinins, Viral |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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