Document Detail

Plaque formation with simian virus 40: enhancement by dimethyl sulfoxide.
MedLine Citation:
PMID:  4372414     Owner:  NLM     Status:  MEDLINE    
Dimethyl sulfoxide (DMSO) added to agar overlays during plaque assays of simian virus 40 (SV40) in CV1 monkey cells increases the plaque size and number and enables plaques to be read several days earlier than usual. DMSO appears to act during development of plaques, perhaps by causing cell lysis at smaller burst sizes in the presence of near-lethal DMSO concentrations. It does not act synergistically in determining virus inactivation with UV light and is equally effective on wild type and a late mutant of SV40.
J E Cleaver
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of virology     Volume:  14     ISSN:  0022-538X     ISO Abbreviation:  J. Virol.     Publication Date:  1974 Dec 
Date Detail:
Created Date:  1975-02-18     Completed Date:  1975-02-18     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1607-10     Citation Subset:  IM    
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MeSH Terms
Cell Line
Dimethyl Sulfoxide*
Plaque Assay / methods*
Radiation Effects
Simian virus 40 / growth & development*,  radiation effects
Ultraviolet Rays
Virus Replication
Reg. No./Substance:
67-68-5/Dimethyl Sulfoxide

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