| Plaque formation with simian virus 40: enhancement by dimethyl sulfoxide. | |
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MedLine Citation:
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PMID: 4372414 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Dimethyl sulfoxide (DMSO) added to agar overlays during plaque assays of simian virus 40 (SV40) in CV1 monkey cells increases the plaque size and number and enables plaques to be read several days earlier than usual. DMSO appears to act during development of plaques, perhaps by causing cell lysis at smaller burst sizes in the presence of near-lethal DMSO concentrations. It does not act synergistically in determining virus inactivation with UV light and is equally effective on wild type and a late mutant of SV40. |
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Authors:
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J E Cleaver |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of virology Volume: 14 ISSN: 0022-538X ISO Abbreviation: J. Virol. Publication Date: 1974 Dec |
Date Detail:
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Created Date: 1975-02-18 Completed Date: 1975-02-18 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0113724 Medline TA: J Virol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1607-10 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Line Dimethyl Sulfoxide* Haplorhini Kidney Plaque Assay / methods* Radiation Effects Simian virus 40 / growth & development*, radiation effects Ultraviolet Rays Virus Replication |
| Chemical | |
Reg. No./Substance:
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67-68-5/Dimethyl Sulfoxide |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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