Document Detail


Plaque formation and purification of BK virus in cultured human urinary cells.
MedLine Citation:
PMID:  6255084     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Primary human urinary cells support growth and plaque formation by papovavirus BK, permitting plaque purification of the virus. After plaquing, the virus forms uniform plaques and its DNA has a homogeneous restriction enzyme fragment profile. Support of BK replication as well as morphological and cultural characteristics suggest an epithelial origin for the cells.
Authors:
G H Sack; J S Felix; A A Lanahan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of general virology     Volume:  50     ISSN:  0022-1317     ISO Abbreviation:  J. Gen. Virol.     Publication Date:  1980 Sep 
Date Detail:
Created Date:  1981-02-26     Completed Date:  1981-02-26     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0077340     Medline TA:  J Gen Virol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  185-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
BK Virus / analysis,  growth & development*,  isolation & purification
DNA, Viral / analysis
Humans
Plaque Assay
Polyomavirus / growth & development*
Urine / cytology*,  microbiology
Virus Cultivation
Grant Support
ID/Acronym/Agency:
CA16754/CA/NCI NIH HHS; CA20619/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/DNA, Viral

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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