Document Detail

Plaque formation by 55 rhinovirus serotypes.
MedLine Citation:
PMID:  4300894     Owner:  NLM     Status:  MEDLINE    
Plaque production by 55 rhinoviruses in several lines of HeLa cells is reported. Forty-nine types produced macroscopic plaques under the conditions described previously employing 30 mM MgCl(2) and 30 mug/ml of DEAE-dextran in overlay and M HeLa cells. Basically three kinds of plaques were observed: clear large and intermediate sized plaques and small turbid plaques. Five rhinoviruses produced plaques only in a sensitive clonal line isolated from the M HeLa line. Rhinovirus type 4 produced plaques after the pretreatment of cells with actinomycin D. Enhancement of plaque formation by MgCl(2) has been demonstrated to date with 17 rhinoviruses. Some rhinoviruses were enhanced either by DEAE-dextran or by dextran sulfate in the overlay. No inhibitors were found in any of 10 bovine sera tested. Rhinovirus type 2 was visualized inside HeLa cells by electron microscopy and there appeared to be a very high ratio of physical particles per infectious unit of virus.
M Fiala
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Applied microbiology     Volume:  16     ISSN:  0003-6919     ISO Abbreviation:  Appl Microbiol     Publication Date:  1968 Oct 
Date Detail:
Created Date:  1968-12-08     Completed Date:  1968-12-08     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7605802     Medline TA:  Appl Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1445-50     Citation Subset:  IM    
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MeSH Terms
Culture Media / pharmacology
Cytopathogenic Effect, Viral* / drug effects
Dactinomycin / pharmacology
Dextrans / pharmacology
Hela Cells
Magnesium / pharmacology
Microscopy, Electron
Rhinovirus / growth & development,  isolation & purification*
Virus Cultivation
Reg. No./Substance:
0/Culture Media; 50-76-0/Dactinomycin; 7439-95-4/Magnesium; 9004-54-0/Dextrans

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