Document Detail


Plaque formation by 55 rhinovirus serotypes.
MedLine Citation:
PMID:  4300894     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Plaque production by 55 rhinoviruses in several lines of HeLa cells is reported. Forty-nine types produced macroscopic plaques under the conditions described previously employing 30 mM MgCl(2) and 30 mug/ml of DEAE-dextran in overlay and M HeLa cells. Basically three kinds of plaques were observed: clear large and intermediate sized plaques and small turbid plaques. Five rhinoviruses produced plaques only in a sensitive clonal line isolated from the M HeLa line. Rhinovirus type 4 produced plaques after the pretreatment of cells with actinomycin D. Enhancement of plaque formation by MgCl(2) has been demonstrated to date with 17 rhinoviruses. Some rhinoviruses were enhanced either by DEAE-dextran or by dextran sulfate in the overlay. No inhibitors were found in any of 10 bovine sera tested. Rhinovirus type 2 was visualized inside HeLa cells by electron microscopy and there appeared to be a very high ratio of physical particles per infectious unit of virus.
Authors:
M Fiala
Related Documents :
11274964 - Role of proteasomal degradation in the cell cycle-dependent regulation of dna topoisome...
24373334 - Visualizing hepatitis b virus with biarsenical labelling in living cells.
341644 - Endocytosis of salmonella typhimurium 395 ms and mr10 by hela cells.
7640664 - Morphological alterations caused by lithium in various cell lines.
21618824 - Latrophilin signalling in tissue polarity and morphogenesis.
7827934 - Proliferating cell nuclear antigen in the assessment of barrett's mucosa.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Applied microbiology     Volume:  16     ISSN:  0003-6919     ISO Abbreviation:  Appl Microbiol     Publication Date:  1968 Oct 
Date Detail:
Created Date:  1968-12-08     Completed Date:  1968-12-08     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7605802     Medline TA:  Appl Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1445-50     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Culture Media / pharmacology
Cytopathogenic Effect, Viral* / drug effects
Dactinomycin / pharmacology
Dextrans / pharmacology
Hela Cells
Magnesium / pharmacology
Microscopy, Electron
Rhinovirus / growth & development,  isolation & purification*
Virus Cultivation
Chemical
Reg. No./Substance:
0/Culture Media; 50-76-0/Dactinomycin; 7439-95-4/Magnesium; 9004-54-0/Dextrans
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  A study of the mechanism of quantal transmitter release at a chemical synapse.
Next Document:  Activities of arginine dihydrolase and phosphatase in Streptococcus faecalis and Streptococcus faeci...