Document Detail

Plant tissue-based chemiluminescence flow biosensor for glycolic acid.
MedLine Citation:
PMID:  11305652     Owner:  NLM     Status:  MEDLINE    
A novel plant tissue-based chemiluminescence (CL) biosensor for glycolic acid combined with flow injection analysis is proposed in this paper. The spinach tissue acts as the molecular recognition element. Glycolic acid is oxidized by oxygen under the catalysis of glycolate oxidase in the tissue column to produce hydrogen peroxide, which can react with luminol in the presence of peroxidase of spinach tissue to generate a CL signal. The CL emission intensity was linear with glycolic acid concentration in the range of 4 x 10(-3)-4 x 10(-6) mol/L and the detection limit was 1.3 x 10(-6) mol/L. The biosensor was stable for about 3 weeks. A complete analysis, including sampling and washing, could be performed in 1.5 min with a relative standard deviation of 1.7%.
B Li; Z Zhang; Y Jin
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Analytical chemistry     Volume:  73     ISSN:  0003-2700     ISO Abbreviation:  Anal. Chem.     Publication Date:  2001 Mar 
Date Detail:
Created Date:  2001-04-17     Completed Date:  2001-05-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1203-6     Citation Subset:  IM    
Department of Chemistry, Shaanxi Normal University, Xi'an, PR China.
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MeSH Terms
Biosensing Techniques
Chemiluminescent Measurements
Glycolates / analysis*
Spinacia oleracea / chemistry*
Reg. No./Substance:
0/Glycolates; 79-14-1/glycolic acid

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