| Plant single cell sampling. | |
| | |
MedLine Citation:
|
PMID: 23073886 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
Plant single cell sampling and analysis allows the determination of solute concentrations in individual cells and tissues. This is particularly important when studying mineral nutrition, where the cell- and tissue-specific distribution of individual mineral nutrients increases a plant's options to store and mobilize these nutrients in response to a changing external availability. In this chapter, some selected single cell sampling and analysis methods are described in detail, and their advantages and possible pitfalls discussed. These methods include pressure-driven extraction of cell contents (cell sap sampling), and the analysis of extracted cell sap through picoliter osmometry (osmolality), energy-dispersive X-ray (EDX) analysis (concentrations of Na, K, P, S, Cl, Ca), and microfluorometry (concentrations of anions and amino acids). In most cases, the extracted cell sap is mainly vacuolar in origin. |
| | |
Authors:
|
Wieland Fricke |
Related Documents
:
|
23289536 - Toxin-antitoxin systems are ubiquitous and versatile modulators of prokaryotic cell fate. 23557686 - Does 4-tert-octylphenol affect estrogen signaling pathways in bank vole leydig cells an... 22949836 - Wogonin induces reactive oxygen species production and cell apoptosis in human glioma c... 23026046 - Sperm-specific glyceraldehyde-3-phosphate dehydrogenase is expressed in melanoma cells. 7972136 - Commitment to apoptosis is associated with changes in mitochondrial biogenesis and acti... 17954606 - The motor activity of myosin-x promotes actin fiber convergence at the cell periphery t... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Methods in molecular biology (Clifton, N.J.) Volume: 953 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2013 |
Date Detail:
|
Created Date: 2012-10-17 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
|
Languages: eng Pagination: 209-31 Citation Subset: IM |
Affiliation:
|
School of Biology and Environmental Science, University College Dublin (UCD), Dublin, Ireland, Wieland02fricke@yahoo.co.uk. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Methods for xylem sap collection.
Next Document: Measurements of cytosolic ion concentrations in live cells.