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Plant single cell sampling.
MedLine Citation:
PMID:  23073886     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Plant single cell sampling and analysis allows the determination of solute concentrations in individual cells and tissues. This is particularly important when studying mineral nutrition, where the cell- and tissue-specific distribution of individual mineral nutrients increases a plant's options to store and mobilize these nutrients in response to a changing external availability. In this chapter, some selected single cell sampling and analysis methods are described in detail, and their advantages and possible pitfalls discussed. These methods include pressure-driven extraction of cell contents (cell sap sampling), and the analysis of extracted cell sap through picoliter osmometry (osmolality), energy-dispersive X-ray (EDX) analysis (concentrations of Na, K, P, S, Cl, Ca), and microfluorometry (concentrations of anions and amino acids). In most cases, the extracted cell sap is mainly vacuolar in origin.
Authors:
Wieland Fricke
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  953     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-10-17     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  209-31     Citation Subset:  IM    
Affiliation:
School of Biology and Environmental Science, University College Dublin (UCD), Dublin, Ireland, Wieland02fricke@yahoo.co.uk.
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