| Plant regrowth from potato shoot tips cryopreserved by a combined vitrification-droplet method. | |
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MedLine Citation:
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PMID: 19827247 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Shoot tips obtained from in vitro potato plants (three cultivars) were successfully cryopreserved by the combined vitrification-droplet method and subsequently regenerated shoots. The effect of apex size, sucrose concentration, preculture duration and cold hardening treatments on viability of cryopreserved shoot tips was studied. The excised shoot tips were incubated, precultured and dehydrated with concentrated PVS2 cryoprotective solution at room temperature, prior to a direct plunge in liquid nitrogen. After rapid rewarming in Murashige-Skoog (MS) liquid medium, shoot tips were plated on semisolid MS medium (3.5 g/l agar) supplemented with 0.4 mg l(-1) gibberellic acid, 0.5 mg l(-1) zeatin, 0.2 mg l(-1) indole-3-acetic acid and 30 g l(-1) sucrose for regrowth. Cryopreserved shoot tips resumed growth within 20 days and regenerated shoots within 30 days. The highest regrowth levels of apices after cryopreservation were 55% recovery for cv. Désirée, 51% for cv. Ostara and 46% for cv. Santé. |
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Authors:
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A Halmagyi; C Deliu; A Coste |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cryo letters Volume: 26 ISSN: 0143-2044 ISO Abbreviation: Cryo Letters Publication Date: 2005 Sep-Oct |
Date Detail:
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Created Date: 2009-10-15 Completed Date: 2009-11-05 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9891832 Medline TA: Cryo Letters Country: England |
Other Details:
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Languages: eng Pagination: 313-22 Citation Subset: IM |
Affiliation:
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Institute of Biological Research, Cluj-Napoca, Romania. halmagyi.a@gmx.net |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cryopreservation
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methods* Cryoprotective Agents / pharmacology Gibberellins / pharmacology Indoleacetic Acids / pharmacology Plant Shoots / drug effects, growth & development* Solanum tuberosum / growth & development*, physiology Sucrose / pharmacology Zeatin / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Cryoprotective Agents; 0/Gibberellins; 0/Indoleacetic Acids; 1637-39-4/Zeatin; 57-50-1/Sucrose; 77-06-5/gibberellic acid; 87-51-4/indoleacetic acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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