Document Detail

Plant regrowth from potato shoot tips cryopreserved by a combined vitrification-droplet method.
MedLine Citation:
PMID:  19827247     Owner:  NLM     Status:  MEDLINE    
Shoot tips obtained from in vitro potato plants (three cultivars) were successfully cryopreserved by the combined vitrification-droplet method and subsequently regenerated shoots. The effect of apex size, sucrose concentration, preculture duration and cold hardening treatments on viability of cryopreserved shoot tips was studied. The excised shoot tips were incubated, precultured and dehydrated with concentrated PVS2 cryoprotective solution at room temperature, prior to a direct plunge in liquid nitrogen. After rapid rewarming in Murashige-Skoog (MS) liquid medium, shoot tips were plated on semisolid MS medium (3.5 g/l agar) supplemented with 0.4 mg l(-1) gibberellic acid, 0.5 mg l(-1) zeatin, 0.2 mg l(-1) indole-3-acetic acid and 30 g l(-1) sucrose for regrowth. Cryopreserved shoot tips resumed growth within 20 days and regenerated shoots within 30 days. The highest regrowth levels of apices after cryopreservation were 55% recovery for cv. Désirée, 51% for cv. Ostara and 46% for cv. Santé.
A Halmagyi; C Deliu; A Coste
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cryo letters     Volume:  26     ISSN:  0143-2044     ISO Abbreviation:  Cryo Letters     Publication Date:    2005 Sep-Oct
Date Detail:
Created Date:  2009-10-15     Completed Date:  2009-11-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9891832     Medline TA:  Cryo Letters     Country:  England    
Other Details:
Languages:  eng     Pagination:  313-22     Citation Subset:  IM    
Institute of Biological Research, Cluj-Napoca, Romania.
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MeSH Terms
Cryopreservation / methods*
Cryoprotective Agents / pharmacology
Gibberellins / pharmacology
Indoleacetic Acids / pharmacology
Plant Shoots / drug effects,  growth & development*
Solanum tuberosum / growth & development*,  physiology
Sucrose / pharmacology
Zeatin / pharmacology
Reg. No./Substance:
0/Cryoprotective Agents; 0/Gibberellins; 0/Indoleacetic Acids; 1637-39-4/Zeatin; 57-50-1/Sucrose; 77-06-5/gibberellic acid; 87-51-4/indoleacetic acid

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