Document Detail

Plant polyphenols effectively protect HaCaT cells from ultraviolet C-triggered necrosis and suppress inflammatory chemokine expression.
MedLine Citation:
PMID:  19723070     Owner:  NLM     Status:  MEDLINE    
Oxidative stress is a common response of epidermal cells to a variety of noxious stimuli such as ultraviolet (UV) radiation from solar light and proinflammatory cytokines from skin-infiltrating leukocytes. Here, we report that two types of plant-derived antioxidants, the phenylpropanoid glycoside verbascoside as well as the flavonoids rutin and quercetin possess protective effects against UVC-induced cell damage and proinflammatory activation. The molecules under investigation were effective against the loss of cell integrity associated with necrosis in doses consistent with their antioxidant activity, whereas they did not significantly oppose UVC-induced proliferation arrest and apoptosis. By contrast, only verbascoside effectively inhibited cytokine-induced release of proinflammatory mediators in a dose-dependent fashion. Verbascoside and its homologue teupolioside dramatically impaired NF-kappaB and AP-1 DNA binding activity. These results suggest that plant polyphenols with antioxidant properties have distinct mechanisms in the suppression of oxidative stress induced in keratinocytes by different stimuli. Verbascoside and teupolioside are hence of potential interest in the protection of the skin from both environmental and inflammatory insults.
Saveria Pastore; Alla Potapovich; Vladimir Kostyuk; Valentina Mariani; Daniela Lulli; Chiara De Luca; Liudmila Korkina
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Annals of the New York Academy of Sciences     Volume:  1171     ISSN:  1749-6632     ISO Abbreviation:  Ann. N. Y. Acad. Sci.     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-09-02     Completed Date:  2009-09-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7506858     Medline TA:  Ann N Y Acad Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  305-13     Citation Subset:  IM    
Laboratory of Tissue Engineering and Cutaneous Physiopathology, Istituto Dermopatico dell'Immacolata, IRCCS, Rome, Italy.
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MeSH Terms
Ajuga / chemistry
Antioxidants / pharmacology
Apoptosis / drug effects,  radiation effects
Blotting, Western
Cell Line
Chemokines / metabolism*
DNA / metabolism
Dose-Response Relationship, Drug
Extracellular Signal-Regulated MAP Kinases / metabolism
Glucosides / pharmacology*
Inflammation Mediators / metabolism
Interferon-gamma / pharmacology
Keratinocytes / drug effects*,  metabolism,  radiation effects
NF-kappa B / metabolism
Phenols / pharmacology*
Phosphorylation / drug effects
Plant Extracts / chemistry,  pharmacology*
Protein Binding / drug effects
Quercetin / pharmacology
Rutin / pharmacology
Syringa / chemistry
Transcription Factor AP-1 / metabolism
Tumor Necrosis Factor-alpha / pharmacology
Ultraviolet Rays
Reg. No./Substance:
0/Antioxidants; 0/Chemokines; 0/Glucosides; 0/Inflammation Mediators; 0/NF-kappa B; 0/Phenols; 0/Plant Extracts; 0/Transcription Factor AP-1; 0/Tumor Necrosis Factor-alpha; 117-39-5/Quercetin; 153-18-4/Rutin; 61276-17-3/acteoside; 82115-62-6/Interferon-gamma; 9007-49-2/DNA; EC Signal-Regulated MAP Kinases

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