Document Detail


Plant growth, metabolism and adaptation in relation to stress conditions. XXVII. Can ascorbic acid modify the adverse effects of NaCl and mannitol on amino acids, nucleic acids and protein patterns in Vicia faba seedlings?
MedLine Citation:
PMID:  19093192     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The adverse effects of either NaCl or mannitol on amino acids, protein patterns and nucleic acids in Vicia faba seeds were investigated. The exogenous addition of 4 mM ascorbic acid to the stressing media in which the broad bean seeds were germinated in combination with either the ionic (NaCl) or osmotic (mannitol) stressor induced significant protective changes in the total amount and in the relative composition of amino acids in general and in proline, glycine, glutamic, aspartic, alanine and serine in particular. It also induced changes in nucleic acids (RNA and DNA) content. These changes occurred throughout the entire period of the experiments (12 days). Separate administration of NaCl or mannitol enhanced the occurrence of particular novel proteins that were not detected in control bean seeds (water medium). Protein banding patterns of broad bean seedlings treated with NaCl or mannitol in combination with 4 mM ascorbic acid showed different de novo protein bands, with different molecular weights, at different stages of seedlings growth, with lower levels or a nearly complete absence of the major stress proteins. The pattern of changes for amino acids and nucleic acids and the range of protein bands extracted from the variously treated broad bean seedlings indicate a positive role of ascorbic acid in the alleviation of the damage effects induced by NaCl and mannitol. The importance of this role in the stress tolerance of broad beans is discussed.
Authors:
M E Younis; M N A Hasaneen; A M S Kazamel
Publication Detail:
Type:  Journal Article     Date:  2008-12-18
Journal Detail:
Title:  Protoplasma     Volume:  235     ISSN:  1615-6102     ISO Abbreviation:  Protoplasma     Publication Date:  2009 Mar 
Date Detail:
Created Date:  2009-03-18     Completed Date:  2009-08-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9806853     Medline TA:  Protoplasma     Country:  Austria    
Other Details:
Languages:  eng     Pagination:  37-47     Citation Subset:  IM    
Affiliation:
Department of Botany, Faculty of Science, Mansoura University, Mansoura, Egypt. ME_Younis@mans.edu.eg
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / drug effects
Antioxidants / pharmacology*
Ascorbic Acid / pharmacology*
Mannitol / pharmacology
Nucleic Acids / metabolism*
Plant Proteins / metabolism*
Seedling / drug effects*,  growth & development,  metabolism
Sodium Chloride / pharmacology
Stress, Physiological / drug effects
Vicia faba / drug effects*,  growth & development,  metabolism
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Antioxidants; 0/Nucleic Acids; 0/Plant Proteins; 50-81-7/Ascorbic Acid; 69-65-8/Mannitol; 7647-14-5/Sodium Chloride

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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