Document Detail


Plant-derived Human Collagen Scaffolds for Skin Tissue Engineering.
MedLine Citation:
PMID:  23298216     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Tissue engineering scaffolds are commonly formed using proteins extracted from animal tissues such as bovine hide. Risks associated with the use of these materials include hypersensitivity, and pathogenic contamination. Human-derived proteins lower the risk of hypersensitivity but possess the risk of disease transmission. Methods engineer recombinant human proteins using plant material provide an alternate source of these materials without the risk of disease transmission or concerns regarding variability. To investigate the utility of plant-derived human collagen (PDHC) in the development of engineered skin, PDHC and bovine hide collagen were formed into tissue engineering scaffolds using electrospinning or freeze-drying. Both raw materials were easily formed into two common scaffold types, electrospun nonwoven scaffolds and lyophilized sponges, with similar architectures. Processing time, however, was significantly lower with PDHC. PDHC scaffolds supported primary human cell attachment and proliferation at an equivalent or higher level than bovine material. IL-1 beta production was significantly lower when activated THP-1 macrophages where exposed to PDHC electrospun scaffolds compared to bovine collagen. Both materials promoted proper maturation and differentiation of engineered skin. These data suggest that PDHC may provide a novel source of raw material for tissue engineering with low risk of allergic response or disease transmission.
Authors:
James J Willard; Jason W Drexler; Amitava Das; Sashwati Roy; Shani Shilo; Oded Shoseyov; Heather M Powell
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-9
Journal Detail:
Title:  Tissue engineering. Part A     Volume:  -     ISSN:  1937-335X     ISO Abbreviation:  Tissue Eng Part A     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-9     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101466659     Medline TA:  Tissue Eng Part A     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
The Ohio State University, Materials Science and Engineering, Columbus, Ohio, United States; willard.37@osu.edu.
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