Document Detail

Plant-derived human collagen scaffolds for skin tissue engineering.
MedLine Citation:
PMID:  23298216     Owner:  NLM     Status:  MEDLINE    
Tissue engineering scaffolds are commonly formed using proteins extracted from animal tissues, such as bovine hide. Risks associated with the use of these materials include hypersensitivity and pathogenic contamination. Human-derived proteins lower the risk of hypersensitivity, but possess the risk of disease transmission. Methods engineering recombinant human proteins using plant material provide an alternate source of these materials without the risk of disease transmission or concerns regarding variability. To investigate the utility of plant-derived human collagen (PDHC) in the development of engineered skin (ES), PDHC and bovine hide collagen were formed into tissue engineering scaffolds using electrospinning or freeze-drying. Both raw materials were easily formed into two common scaffold types, electrospun nonwoven scaffolds and lyophilized sponges, with similar architectures. The processing time, however, was significantly lower with PDHC. PDHC scaffolds supported primary human cell attachment and proliferation at an equivalent or higher level than the bovine material. Interleukin-1 beta production was significantly lower when activated THP-1 macrophages where exposed to PDHC electrospun scaffolds compared to bovine collagen. Both materials promoted proper maturation and differentiation of ES. These data suggest that PDHC may provide a novel source of raw material for tissue engineering with low risk of allergic response or disease transmission.
James J Willard; Jason W Drexler; Amitava Das; Sashwati Roy; Shani Shilo; Oded Shoseyov; Heather M Powell
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2013-02-19
Journal Detail:
Title:  Tissue engineering. Part A     Volume:  19     ISSN:  1937-335X     ISO Abbreviation:  Tissue Eng Part A     Publication Date:  2013 Jul 
Date Detail:
Created Date:  2013-05-29     Completed Date:  2014-01-03     Revised Date:  2014-07-01    
Medline Journal Info:
Nlm Unique ID:  101466659     Medline TA:  Tissue Eng Part A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1507-18     Citation Subset:  IM    
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MeSH Terms
Biocompatible Materials / chemistry*
Cells, Cultured
Collagen / chemistry*
Materials Testing
Plants / chemistry*
Tissue Engineering / methods*
Tissue Scaffolds / chemistry*
Reg. No./Substance:
0/Biocompatible Materials; 9007-34-5/Collagen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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