Document Detail


Plant cell proliferation inside an inorganic host.
MedLine Citation:
PMID:  16949175     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In recent years, much attention has been paid to plant cell culture as a tool for the production of secondary metabolites and the expression of recombinant proteins. Plant cell immobilization offers many advantages for biotechnological processes. However, the most extended matrices employed, such as calcium-alginate, cannot fully protect entrapped cells. Sol-gel chemistry of silicates has emerged as an outstanding strategy to obtain biomaterials in which living cells are truly protected. This field of research is rapidly developing and a large number of bacteria and yeast-entrapping ceramics have already been designed for different applications. But even mild thermal and chemical conditions employed in sol-gel synthesis may result harmful to cells of higher organisms. Here we present a method for the immobilization of plant cells that allows cell growth at cavities created inside a silica matrix. Plant cell proliferation was monitored for a 6-month period, at the end of which plant calli of more than 1 mm in diameter were observed inside the inorganic host. The resulting hybrid device had good mechanical stability and proved to be an effective barrier against biological contamination, suggesting that it could be employed for long-term plant cell entrapment applications.
Authors:
Mercedes Perullini; María Mercedes Rivero; Matías Jobbágy; Alejandro Mentaberry; Sara A Bilmes
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-07-31
Journal Detail:
Title:  Journal of biotechnology     Volume:  127     ISSN:  0168-1656     ISO Abbreviation:  J. Biotechnol.     Publication Date:  2007 Jan 
Date Detail:
Created Date:  2006-12-04     Completed Date:  2007-02-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8411927     Medline TA:  J Biotechnol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  542-8     Citation Subset:  IM    
Affiliation:
INQUIMAE-DQIAQF, Universidad de Buenos Aires, Ciudad Universitaria, Pab. II, C1428EHA, Buenos Aires, Argentina.
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MeSH Terms
Descriptor/Qualifier:
Cell Culture Techniques*
Cell Proliferation*
Cells, Immobilized / cytology
Phloem / cytology*
Silicon Dioxide*
Tobacco / cytology*
Chemical
Reg. No./Substance:
7631-86-9/Silicon Dioxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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