Document Detail

Plant cell biology through the window of the highly synchronized tobacco BY-2 cell line.
MedLine Citation:
PMID:  10728644     Owner:  NLM     Status:  MEDLINE    
Synchronous cell systems are highly desirable for investigating various aspects of plant cell biology. However, to date, the tobacco BY-2 cell line is the only plant cell line which can be synchronized to high levels. A cell synchrony starting from S phase is obtained after release of BY-2 cells from aphidicolin treatment, while that from M phase is available after release from a sequential treatment of aphidicolin followed by propyzamide. A high level of synchrony is only attained by using rapidly growing tobacco BY-2 cells that propagate ca. 100-fold in a week. Reduced levels of synchrony result if the growth rate becomes lower. Technical notes for maintaining the high growth rate of the tobacco BY-2 cell are described. Using this highly synchronous system it has been possible to demonstrate the cell cycle-dependent oscillation of many genes, such as cyclins, and characterize their role during the cell cycle. Furthermore, this system has facilitated the structural and biochemical analysis of cell cycle specific events such as the development of the phragmoplast and the formation of cortical microtubules. Other potential uses of this highly synchronized cells are also described.
T Nagata; F Kumagai
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Methods in cell science : an official journal of the Society for In Vitro Biology     Volume:  21     ISSN:  1381-5741     ISO Abbreviation:  Methods Cell Sci     Publication Date:  1999  
Date Detail:
Created Date:  2000-04-20     Completed Date:  2000-04-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9515839     Medline TA:  Methods Cell Sci     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  123-7     Citation Subset:  IM    
Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Japan.
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MeSH Terms
Cell Line / metabolism
Genes, cdc / physiology*
Plants, Toxic*
Time Factors
Tobacco / metabolism,  physiology*

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