Document Detail


Planar cell polarity controls directional Notch signaling in the Drosophila leg.
MedLine Citation:
PMID:  22736244     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The generation of functional structures during development requires tight spatial regulation of signaling pathways. Thus, in Drosophila legs, in which Notch pathway activity is required to specify joints, only cells distal to ligand-producing cells are capable of responding. Here, we show that the asymmetric distribution of planar cell polarity (PCP) proteins correlates with this spatial restriction of Notch activation. Frizzled and Dishevelled are enriched at distal sides of each cell and hence localize at the interface with ligand-expressing cells in the non-responding cells. Elimination of PCP gene function in cells proximal to ligand-expressing cells is sufficient to alleviate the repression, resulting in ectopic Notch activity and ectopic joint formation. Mutations that compromise a direct interaction between Dishevelled and Notch reduce the efficacy of repression. Likewise, increased Rab5 levels or dominant-negative Deltex can suppress the ectopic joints. Together, these results suggest that PCP coordinates the spatial activity of the Notch pathway by regulating endocytic trafficking of the receptor.
Authors:
Amalia Capilla; Ruth Johnson; Maki Daniels; María Benavente; Sarah J Bray; Máximo Ibo Galindo
Related Documents :
9665064 - Cell cycle progression: new therapeutic target for vascular proliferative disease.
10475264 - Exposure to hyperbaric oxygen induces cell cycle perturbation in prostate cancer cells.
8619314 - A double flow cytometric tag allows tracking of the dynamics of cell cycle progression ...
16153144 - Fibronectin-mediated adhesion rescues cell cycle arrest induced by fibroblast growth fa...
17167534 - Higher expression and activity of metalloproteinases in human cervical carcinoma cell l...
1310224 - Regulation of intracellular ph in alveolar epithelial cells.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  139     ISSN:  1477-9129     ISO Abbreviation:  Development     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-06-27     Completed Date:  2012-09-04     Revised Date:  2014-02-20    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  England    
Other Details:
Languages:  eng     Pagination:  2584-93     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adaptor Proteins, Signal Transducing / genetics,  metabolism
Animals
Cell Polarity / genetics,  physiology*
Drosophila
Drosophila Proteins
Frizzled Receptors / genetics,  metabolism
Mutation
Phosphoproteins / genetics,  metabolism
Receptors, Notch / genetics,  metabolism*
Signal Transduction / genetics,  physiology
rab5 GTP-Binding Proteins / genetics,  metabolism
Grant Support
ID/Acronym/Agency:
G0300034//Medical Research Council; G0800034//Medical Research Council
Chemical
Reg. No./Substance:
0/Adaptor Proteins, Signal Transducing; 0/Drosophila Proteins; 0/Frizzled Receptors; 0/Phosphoproteins; 0/Receptors, Notch; 0/dishevelled proteins; EC 3.6.5.2/rab5 GTP-Binding Proteins
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Evolutionary crossroads in developmental biology: hemichordates.
Next Document:  Six3 cooperates with Hedgehog signaling to specify ventral telencephalon by promoting early expressi...