Document Detail


Placentation in the alpaca Lama pacos.
MedLine Citation:
PMID:  12802689     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Reproduction in South American camelids is poorly studied. To extend our knowledge of the development and cellular physiology of the placenta in the alpaca Lama pacos, we have examined specimens from day 150 of pregnancy to term. Morphological investigations using light, transmission and scanning electron microscopy, the histochemical localization of iron, alkaline and acid phosphatase activity, and the immunodetection of placental lactogen hormone were performed. Throughout pregnancy there was a progressive increase in the depths of folds on the uterine mucosa surface together with a thickening of the endometrium. Glandular cells exhibited PAS and acid phosphatase (AcP) positive secretion granules. In the chorion, giant trophoblast polyploid cells gradually became more numerous and larger. Non-giant cells exhibited positive granules for PAS, alkaline phosphatase (AkP) reaction and immunostaining for bovine placental lactogen hormone (PLH). SDS -PAGE electrophoresis and Western blotting procedures also confirmed the presence of a bovine PLH-like glycoprotein in the fetal alpaca placenta. Over the glandular openings, the chorion formed typical areolae, where the trophoblast exhibited AcP and PAS positive reactions. At these sites, the fetal endothelial cells contained iron-storage granules in their cytoplasm. The trophoblast-epithelial interface exhibited a complex microvillous interdigitation, in which an AkP reaction was very prominent. The chorionic capillaries progressively indented adjacent trophoblast cells. These data suggest that although the epitheliochorial alpaca placenta is diffuse, various trophoblast cell types and specialized areas of the maternofetal interface give the placenta micro-regional functions where histiotrophic nutrition, hormone production and molecular exchange are prevalent.
Authors:
Luis Olivera; Douglas Zago; Rudolf Leiser; Carolyn Jones; Estela Bevilacqua
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2003-06-11
Journal Detail:
Title:  Anatomy and embryology     Volume:  207     ISSN:  0340-2061     ISO Abbreviation:  Anat. Embryol.     Publication Date:  2003 Jul 
Date Detail:
Created Date:  2003-07-07     Completed Date:  2004-03-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7505194     Medline TA:  Anat Embryol (Berl)     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  45-62     Citation Subset:  IM    
Affiliation:
Faculty of Veterinary Medicine & Zootechnology, National University Altiplano, 291 Puno, Casilla, Peru.
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MeSH Terms
Descriptor/Qualifier:
Acid Phosphatase / metabolism
Alkaline Phosphatase / metabolism
Animals
Camelids, New World / anatomy & histology*,  metabolism
Capillaries / metabolism,  ultrastructure
Cell Size / physiology
Chorion / blood supply,  metabolism,  ultrastructure
Endometrium / metabolism,  ultrastructure
Epithelial Cells / metabolism,  ultrastructure
Female
Immunohistochemistry
Metrial Gland / metabolism,  ultrastructure
Microscopy, Electron
Microscopy, Electron, Scanning
Microvilli / metabolism,  ultrastructure
Placenta / growth & development*,  metabolism,  ultrastructure*
Placental Lactogen / metabolism
Pregnancy / physiology*
Trophoblasts / metabolism,  ultrastructure
Chemical
Reg. No./Substance:
9035-54-5/Placental Lactogen; EC 3.1.3.1/Alkaline Phosphatase; EC 3.1.3.2/Acid Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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