| Placental protein 13 (PP-13): effects on cultured trophoblasts, and its detection in human body fluids in normal and pathological pregnancies. | |
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MedLine Citation:
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PMID: 15193867 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Placental tissue protein 13 (PP-13), one of the 56 known placental proteins identified till today, was purified from placentas obtained from women at delivery, and used to evoke antibodies against it. The purified PP-13 was lysed to peptides, which were sequenced, leading to the full-length cDNA sequencing and its expression in Escherichia coli. Sequence analysis in databases showed homology to the galectin family. Of the various antibody preparations developed, a pair of monoclonal antibodies (MAbs) coupled to the recombinant PP-13 (PP-13-R) was used for the immunodetection of PP-13 in pregnant women's serum with the solid-phase ELISA format. With a dynamic range of 25-500 pg/mL with no background in non-pregnant women's serum and men's serum, the ELISA test was suitable for the detection of PP-13 in the 1st, 2nd, and 3rd trimesters. PP-13 levels slowly increase during pregnancy. In the 1st trimester, lower than normal PP-13 levels were found in fetal growth restriction (IUGR), preeclampsia (PE), and particularly in early PE (<34 weeks of gestation). In the 2nd and 3rd trimesters, higher than normal concentrations were found in PE, IUGR and in preterm delivery (PTD). Application of PP-13 to cultured trophoblasts elicited depolarization carried by calcium ions, followed by liberation of linoleic and arachidonic acids from the trophoblast membrane, and a subsequent elevation of prostacyclin and thromboxane. These effects were negligible when PP-13 derived from the placentas of patients with IUGR, PE or PTD was used. The results are discussed in view of the potential utilization of PP-13 for early serum screening to assess the risk to develop placental insufficiency, coupled to a differential analysis of the various pathologies by analyzing cultured trophoblasts. |
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Authors:
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O Burger; E Pick; J Zwickel; M Klayman; H Meiri; R Slotky; S Mandel; L Rabinovitch; Y Paltieli; A Admon; R Gonen |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Placenta Volume: 25 ISSN: 0143-4004 ISO Abbreviation: Placenta Publication Date: 2004 Aug |
Date Detail:
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Created Date: 2004-06-14 Completed Date: 2004-12-23 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8006349 Medline TA: Placenta Country: England |
Other Details:
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Languages: eng Pagination: 608-22 Citation Subset: IM |
Affiliation:
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Diagnostic Technology, 49 Ha'Histadrut St., PO Box 25266, Haifa 31250, Israel. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Amniotic Fluid / chemistry Animals Antibodies, Monoclonal Base Sequence Body Fluids / chemistry* Cells, Cultured DNA, Complementary / analysis, chemistry Enzyme-Linked Immunosorbent Assay Female Fetal Growth Retardation / metabolism Galectins Gestational Age Humans Mice Mice, Inbred BALB C Molecular Sequence Data Obstetric Labor, Premature Pre-Eclampsia / metabolism Pregnancy Pregnancy Complications / metabolism* Pregnancy Proteins / analysis*, genetics, pharmacology* Radioimmunoassay Recombinant Proteins Sensitivity and Specificity Sequence Homology Trophoblasts / drug effects* |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/DNA, Complementary; 0/Galectins; 0/LGALS13 protein, human; 0/Pregnancy Proteins; 0/Recombinant Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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